Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 277728
End position: 278168
Length: 441

DNA sequence
>NC_016510, from 277728 to 278168 (441 bp); Flavobacterium columnare ATCC 49512 TTAATCCCAATCAGCGGCAACAAAACGCATTTTCTTTTTTTCATTTATAAAAAGTTCTAATTGATGTGCT TTTGTAGCGTTAAAACTATTAACTTTTGGTATTAATTTTGCAAAAATTTCTTCGATTTTTGCGGTTTCTT CACAAAACATTTTAGTTGAAATAATATTCGTAGCAGAAAAAGTATTAGAGTTTTTAAATTGATAACTAAA ATTAAATTTATTACAACCTAAGTAGGCTGATGCTTCTCCATTTTTTATAGTCATATCAATATACGCATTA TTTCTTATAAGTATTTCAGAAGGGATTGAATCCATTTCTACAAGCATCCATTTTCTGTTAAGGAGCCTTT CTTTTTCTTTAATTACTTTAGAGTGGCAAGAAAAAAAGAAAACAATAAAAGTTAAAACGATGCTTTTTTT TATTAGTTTTTTAAATATCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 277728-278168	Sequence	FCOL_01140 - hypothetical protein (Protein is coded in complementary strand; from 278168 to 277728)
ORF... 278158-278610	Sequence	FCOL_01145 - AsnC family transcriptional regulator (Protein is coded in complementary strand; from 278610 to 278158)