Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 252117
End position: 252755
Length: 639

DNA sequence
>NC_016510, from 252117 to 252755 (639 bp); Flavobacterium columnare ATCC 49512 TCAATCAAAATTCGTTAATCTTAAATCATCAATCAGTAACCTACATTTCTCCACATCTTTCAACCCTGCA TCGATTTCAAATTTACTATTGACGTCTATGGCGTAACATTTTTGACCGACGTCAGTTTTTAGGAAAGCTT TTAGGGTGTCTAATTCGTTGATGCCAATACCTCCACTTAAAAAATAAGGTTTATCGGAAGGATAATTTTT TAAAATATCCCAATCAAAAGTAAATCCATTGCCTCCGTGTTGTTGTCCTTTGGTGTCAAACAAATAATAG TCGCAAACATTTTCATAGGCTAACAAACTTTGAAAATCAAAATGAGCATCCATCGAAAATACTTTGATAA CTTCAATTGAAGTGTCTTTTAAGTCTTGGCACATCGTTGCAGACTCTTTTCCGTGTAATTGCACTGCATT CAATTGATGCTGTTGTATTTTTTGTTGAATGGTTTCCAAATCAGCATTGACAAACACGCCCACTTTTTTG ATGGTATTGGGAATCTCAGGGATATTTCCTGTGCAATGCCTTGGAGACATTTCATAAAAAATAAAACCTA AATAATCGGGTTTTAGTTTCGAAACTGCCTGTATGTTGACAGGGTATTTCATTCCGCATATTTTGATTTT TATTGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 252117-252755	Sequence	FCOL_01030 - phosphoribosylanthranilate isomerase (Protein is coded in complementary strand; from 252755 to 252117)