Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 2293923
End position: 2296265
Length: 2343

DNA sequence
>NC_016510, from 2293923 to 2296265 (2343 bp); Flavobacterium columnare ATCC 49512 ATGAAAATAATAGGTGATATAAGACCATATACAAACAAAAATTACAGCTATACAGTAGTTGATAATGACG GAGGAACAACATTTGTAATTCTTTGGCAAGTATATAACAATGCTAAATTGCTTACCGAAAATGGAACTGG GATTTTTAAGTTTGGAATTAATACAGTTGGAAATACCTTTACACTAATTGCCAAAGTAAGAAATCCTGAA ACCAATAAAATAGAAGATATAGCAACTGAAATACAACCCCTAGCAGGAAAACCCGAAATACTCGACCTTT ATTGGAGAGATGTAAATGGAGAAAAAATAGATCATAGAGAAGTGGCTTATCTAGACAAAGTCACCTTAGT AATAAAAACCAAAAATATTCCCCAAGGGGACATCCTTAAAATAACGATTTTTGAAGATGAATACCTAGAC GGTCATGGAGATAGCTCTCGGAATATGGGAAAATACTACACTAAAGGCGTCACAAAGAACGGCTACGCTT ACCTAGACCTTGCCAATATGAGTCTTTACCAAAAAAAACTCAATAACAGTGATTATGTAAATGATGAAGT ACACTTGTTTTACGCACAAATACACTATTACAATAAGTTAAACCAGATAAAAGACCAAATACAAATAAAG GTAAATAATGAACTCACACAAATGATACAACCCTATGCAGGCAATAATCCTGCGAAAATAGGCCAAGTAG AAAACAAAGCAAAACAAAGAAAGAAAGCCGTACATTTTACCTTTGGTGTTTTTTTAGATGGTACCCTAAA CAATATGTATGATACGGAACTAAGACAGTATGCAGAAGGAAAAAAAGTAATCAATACCAGTGGTTTAGGT GTAAGCCAATCTCGAGCTATAGATATTTATCAAGACCACGGCGATCCTGATGAAATGCAAAGTAGTTATG AAAACGACCTCTCCAATCCCGCTATATTGTATAAGAATTATATTTCTAAATTAGAAGAAAAAAGATTTAG AATTTATATAAGCGGTATAGGTACCAATACAGCTCCTAAAAACCAAGGGCAAACCTTGCGGCCAGAGGAT TATAAAGACAACGACCTCATAGAAGGTCCCGCTTTTGGTATGGGCAGTTCAGGTATTATGGATAATGTAA AAAAAGCCATTTTAGACGTTGTAAAAGAATTAAAAAATATTAAATCAGATCAAAAAATAGGAACTATAAC TTTTGACGTATTTGGTTTTAGTCGTGGTGCCGCTGCCGCACGGCATTTTGTACACATAGTTACACATCCT CCCTACAATCCTATTGTGTACAAAAAAAAGGAGCGTGACTCGATTGTATTAGACTTGCAAAAGAATCAAT TGCCTGTTTCTTATAAAAACAAAACCATGCCTCATTTTGGACTCCTAGGGCAGTTGCTTCAAGAAAACGG GATTTTAGATGCACAAACACAAGTTAACGTTCGTTTTGTAGGTATTTATGACACCGTTTCGCATCATGGG CTATTTCAGGATAATGATATAAAAGACCTAGGATTGGATAGTGTAAACAAAGCAGATTATGTTGTACACA TGGTAGCAGCAGATGAGCATCGTGCTAATTTTAGTTTAGCAGATATTTCTTCTGTTGCTAAAACCCATCC TGAAAGTGGCAAAAAAGGAGGAATAGAGCTCATCTACCCTGGAGTACATTGCGATGTAGGAGGCGCTTAT GAAGATGGTAAACCAGATAATCCTAAAAGAATTGCGGTAGAATGGGATCTTCCTTCTTTAAAAAAAGTAA GAGAGGAACTCATCAGACAAGGTTGGTTTAAAGAAGATCAACTGTTTGTGAAATTTCAAAATGGATGGGA CTTAAATGCGAATGCACGTAAATGCTATTTAGAGGGCAACAGAAAATGGCTTAGTAATCAGTACAGCTAT ATCCCTCTGCATATTATGGCAAAAATTTGTGGTATAAAAAAGGTGCAGATAAATAATAAACAATTATTAG AATTTAAAAATTTTAAAGAAAATACCTATAATGATCCCAAAGCGCATACTTTAGGAAATCATATAGTTGG AAATATAGCCTTTTTAGAACGGATCAAAAAAAAGCTAGAAGATTATACGTTTAATGGGGGGATGCCTTTT GGTTTTATAGAATACAAAGAACCCGCTACAATATATGAAACAGATGCCTCTAAGGCAAGTGTGAATAAAG AGCGTGAAGAACGCGAAAAACAAAAAGAAGAAGAAATAAATAAAGATATTAGATTCCTACGCAACCACTA TTTGCACTGGAACGCTACTTATGGTAGTTCAGGGATAGATCTTTTGGTAGAAAAAGATTACCCGAATATA ATAAATGGTAAAAGAAAAAGAAATGTATACTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2293923-2296265	Sequence	FCOL_09780 - hypothetical protein
ORF... 2296237-2297301	Sequence	FCOL_09785 - hypothetical protein