DNA sequence >NC_016510, from 2047101 to 2047324 (224 bp); Flavobacterium columnare ATCC 49512 TTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTC TTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTC TTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTC TTTACTTTTTACTC Translate to protein Restriction digest BLAST Design primers with primer3