DNA sequence >NC_016510, from 2047100 to 2047323 (224 bp); Flavobacterium columnare ATCC 49512 ATTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACT CTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACT CTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACT CTTTACTTTTTACT Translate to protein Restriction digest BLAST Design primers with primer3