DNA sequence >NC_016510, from 2047099 to 2047322 (224 bp); Flavobacterium columnare ATCC 49512 AATTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTAC TCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTAC TCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTACTCTTTACTTTTTAC TCTTTACTTTTTAC Translate to protein Restriction digest BLAST Design primers with primer3