Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 2002435
End position: 2003745
Length: 1311

DNA sequence
>NC_016510, from 2002435 to 2003745 (1311 bp); Flavobacterium columnare ATCC 49512 TTAAATCATTTTCTTTTTCTTTTTAATAAACTCCTCTTTGATTTTATCATTTTGCAAATTACCAATACTT CCTTCGTGGGTATGGTTTAGCGTTTCATAATTATAGATAAACTGATTTTCTTCCACTTGTTTTGAAACTA TTTTATAGGCTTCTCTGAACGGAATACCGGATAAAACCAATTCGTTAACGGCTTCAACCGTGAATAGATT CAAATATTTTTCGTCTGATAGAATGTTTTGCTTAATGGTAATATTTTCTAACATTACTTGAAGCATGTCT AAACATTCTTTTAAGGTGATAATCGCTGGGAATAAACATTCTTTGGTTAATTGCATATCCCTGTGATACC CCGAGGGTAAATTGTTAGTCAATAACGTTAGCTCATTAGGAAAAGCTTGAATTCTGTTGCATTTGGCTCT AATCAATTCGAAAATATCTGGATTTTTTTTGTGCGGCATGATACTACTTCCTGTGGTTAATTCTTTAGGA AAACTAATAAATCCGAAATGTTGATTCATGTACAAACACACGTCCATTGAAAATTTTGTGAGTGTAGCTG CTATTCCAGATAAAGCCATAGCAGTTGTTTTTTCCATTTTTCCTCTTGTCATTTGAGCATAGACCACATT ATGATTCATCGATTCAAAATTGAGTAGCAAGGTGGTGAATGTTCTATTTAAAGGAAAAGAAGAACCATAA CCAGCACCAGATCCTAAAGGATTTTTATTAGCCATAGCATAGGCCGCTAAAATCAGTTCTAAATCATCAC TTAAGCTTTCTGCATAAGCGCCAAACCACAAACCAAACGAGGAAGGCATTGCTAATTGAAAATGCGTATA GCCTGGTAATAACTCGTTTTTATGTTTCTCACTTAAATCAATCAAAGTATTAAAAACAGTTTCGGTCTTT TCGACAATTTGTTTCAATTCTGATTTTAGAAACAACTTAATATCCAACAATGATTGATCGTTTCTGGAAC GACCGGCATGGATTTTTTTTCCTACCTCTCCTTTCATTTGAGTTAGTAACAATTCGACTTGGGAATGCAC ATCTTCTACACCCTCTTCAATTACAAATGACTTATCAAGTACCATTTTCAGAATGGATTGCAACCCCTCG TGGGCTTCTTTTAGTTCTTCAGTAGTTAATAATCCTATTTCATGCAACATAGATGTATGAGCCAAAGAAC CTTTGATATCAGCTTCTGCCAAATATAAATCCCAAATAGTATCTTGGCCTATCGTAAATTGTTCCACCAT TGCTGAAATGGAAGTTTTTACGTTATCTGATGTATTTTGCCACAACTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 2001977-2002438	Sequence	FCOL_08635 - AsnC family transcriptional regulator
ORF.. 2002435-2003745	Sequence	FCOL_08640 - argininosuccinate lyase (Protein is coded in complementary strand; from 2003745 to 2002435)
ORF... 2003717-2004811	Sequence	FCOL_08645 - peptidase M20 (Protein is coded in complementary strand; from 2004811 to 2003717)