Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 1968620
End position: 1970017
Length: 1398

DNA sequence
>NC_016510, from 1968620 to 1970017 (1398 bp); Flavobacterium columnare ATCC 49512 TTAGTTTTGATTATCTCTTTTATCATTTCTGTTTTTTGACCTATTATTTTTGGGTCTTGGTCTATTATTT TTAGGCCTTTGCCCTTCTGTTTGACTATTTTTATTCAAATCTCCTTTAGGATGTATAACTTCTGACTTAG GTTTAATACCCCCTTCTGGTTTGGGTTTGATTCCTTCTGGTTTGGACTTATGCTTATTGTTCTCAAAACG AGGTTTTATCGTTTTTTCTATTTTATTTTCAATAGGTTTTTCAGCAGAAACAGTAGAAGTACTTATTTTC TTTTTTTTATTTTTATTTTTTTTCTTAGGCTGGTCAAAACGGGTTAGACTGTCTTGTCCCATGGCATTTT GAAATGTTTTTTCAACTGTTTCTTTTTCTTCCTCGTAGACAAAGTCTTCTAAAGTGGCTACTCTTTTTCC CTGCTTGTTTAAGTCTATAATTTCTTTTACTTCACTTACCTTAAGCATGTGCCAAATAGCCATGTTATCG GGATAAGCAAACCACATTAGTTCTTTGAATATATCAATTTTTTGGCAAGTGGCTTCTCCTCTATCCGTTT GTAATTTGGTTTCCATATCTGGAAAATCCTTTAAGGCATCCATATAGGTGTCTAACTCATAGTTTAAACA ACATTTAAGTTTACCACACTGACCCGCTAATTTTTGGGGATTTAAAGAAAGTTGTTGATAACGTGCTGCT GAAGTATTTACACTTCTAAAATCTGTTAACCAAGTAGAACAACACAATTCACGTCCACAAGAGCCTACTC CCCCTAAACGAGCGGCTTCTTGACGGAAACCCACTTGTTTCATTTCTATTCGGATTTTAAATTCCGCTGC ATAATCTTTAATTAATTGTCGAAAATCCACACGGTCGTTGGCCGTGTAATAAAACGTGGCTTTAGAACCA TCACCTTGAAATTCGATATCAGAAATTTTCATTTCTAAATTTAAAGCAATGGCTAATTCGCGTGCTTTAA CTTGAATACTTGCTTCTTTATTTCTAACTTCTTGCCATATATCAATATCCTTTTGTGAAGCTTTACGGTA AATTTTAGGTACTTCTCCTGCATAATCTACTCCCTTTTTCTTCATTTGTACTTTAACCAATTCTCCTGTT AAAGTAACTATTCCAACGTCATGCCCGGGATTGGCTTCTGTAGCTACTATATCACCAATAGATAGGCTTG TTTTTTCAGGGTTTCTAAAAAACTCTTTTCTACCATTTTTAAAACGAACTTCTACACAGTCAAAAGACTC TTGTCCACCAGGAAGAGCCATATTAGCTAACCAATCAAAAACGGTTAGTTTATTGCAACTGTCTGTGCCA CAGGTGCCATTATTTTTACACCCCTTAGGCGCACCGCCCGTAGAGGTTGAACAACTTGTACATGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1968142-1968645	Sequence	FCOL_08475 - gliding motility protein GldH (Protein is coded in complementary strand; from 1968645 to 1968142)
ORF.. 1968620-1970017	Sequence	FCOL_08480 - hypothetical protein (Protein is coded in complementary strand; from 1970017 to 1968620)