Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 1319241
End position: 1319858
Length: 618

DNA sequence
>NC_016510, from 1319241 to 1319858 (618 bp); Flavobacterium columnare ATCC 49512 CTACAAAGTATAGTAAATACCTACGTTAAATCTATTTCCTAGAGAATTAATATCAAAATTAAACGTATTC TTTTCTTCCTTTACATCCGTTTCCTTATTCTTTGATGTATGCGATGAAAAACCTAAAACACCTAAAGAAC CTACTACTGTAATTTTAGGAGAAAGAGCATAACCAAATCCTGCATTTAGATTTGTATTAAGTTCAAAAGT GGTATTTTTAGATTCTAGATTCCCTAAAATAGTTTTTCTAGTAGTAGTAGAGTTTCCAGGTAACAATCCA ATGTTAAGTCCTACAAAAGGTCTAAAAGCCTTGCTCCCTTCTCCAAAAAAATAACGACCATAAAGAGTAC CTCCATATTGATTTTCTTTTATTTCTGTATTTCCAGATTCTTTTGTTGAACCTTGAGTTGTAAAGCCCAC ACCTAATTGTACATTATTTGTTAAAAAAGTACCTACTTCTGGTGAAAAGTTCCACGTTGAACCCTCTGAT GTAGTTGTACTCTCTATTTTTGTTTTACTACTTGAATAGCCTACTTGACCACCTACATAAAAACTCCCTT TTTGAGCAAATACCCCTGAGGTAATTAATATTGCGCTTGCAATTAAAAAATTTTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1319241-1319858	Sequence	FCOL_05690 - hypothetical protein (Protein is coded in complementary strand; from 1319858 to 1319241)