Sequence retrieval

Genome: Flavobacterium columnare ATCC 49512
Start position: 1275580
End position: 1276464
Length: 885

DNA sequence
>NC_016510, from 1275580 to 1276464 (885 bp); Flavobacterium columnare ATCC 49512 TTATAATTTGTTGATTTCTTCTTTGGTTAAGCCTGTTGCCTGCGTAATGATTTCTACTGAAACACCTAAT CTTTTTAAGGATTTAGCCGTTTCAATCTTTCCTTCAATCTTTCCTTCAATCTTTCCTTCAATCTTTCCTT CAATTAATCCTTCCATCTTTCCATCGTCGAAAGCAGTGTCGATAACGCCTTTTAGATCTCGGTAAACTTT AAGACTGCTCTCGTATTTGTCTAAATCTAATTGCCCAAAATTTGCCAATTCTGCTTTTTCAAAGGCTTGG GTAAATACTTCGTCTTTGAAAATTGTGGGAATATTTTGAAAATCTTCCAAGTTTTTGATGAAATATAACC ATTTGTCTAAACGGGAATTTAATTGGTTTTCCTGTTTGTTAAAATTGGGCATTTCCAAGTAGATATAGGT CAGTTTATCGTAAAATACTTTTCCGTTTTGGTTTTTCAGCTTGATGGTATGCACTACCTCGCTTCTTTCG GGTTCGCTTTCGTAATCGTCGAAAGTAAAATCCAAAACACCTACGCAATAAACCGCTTTCAAATTATAGT TCCATTCTCCTTTTTCGGCTTGTTCTCTAATGGGAAATGTGGAGTAATAGATGGTTCTTTCTTTGAAGTA GTTTTGCTTGGCTTTTTGGAGTTCTACGATGAATTTTTCGCCTTTTTCGTTTTCGCAATAGATGTCGTAG ATGGCTTTTCTGTCGAGGTCACTCTGCCCGAGTTGGTCGGTGTTTTTAAAAGAAAGTTCTTTGATCTGGT CATATTCTGGCAACAAGGCATTGAGGAAGTCGATAAGCAGGTTTTTACTGGCTTCTTCCCCAAATATCTT CTTAAACCCAAAATCCGTAAAGGGATTGATGTATTTTGCCTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1275580-1276464	Sequence	FCOL_05505 - hypothetical protein (Protein is coded in complementary strand; from 1276464 to 1275580)