Sequence retrieval

Genome: Acidithiobacillus ferrivorans SS3
Start position: 279853
End position: 281607
Length: 1755

DNA sequence
>NC_015942, from 279853 to 281607 (1755 bp); Acidithiobacillus ferrivorans SS3 ATGCCACCGGAACGACTAAGCCTGCAACGCATTTTGCCGCGCTGGCGGGCGACCCTGCTCCTTATCGTCA TTCTGCTGGGGCTGGTGGTGGTGCTGACACGAGATGCAGAATTGCAGTGGTGGCGGGCCCATGATTTGCG TGCCCAAGGGCGCATGCGTTATTTGCAGACGCGGCCGTTGCCCGCCGGCCGGGGCGCGATCTATGGCAGC AACGGCGATGCGCTAGCGGCAAATGTGCCAGCGGTGACCATCTGGACCGATCCGCGCCTCTTTAATGCCC ACCGCAAAGATTGGGACAAGGTGGCGCAGGCTCTGGACATCAGTCCGGAGACTCTGGCGGCGCGGGTGCA GTCGGGTGGGTCAGGCTTCGCCTATATCCTGCGTCAGGTGGAACCGCAGTTGGGTAAGTCTCTGGACGCG CTGCATGTGCCCGGTATCTATGTGCAAAAAACCAGCCGCACCTATTATCCTCTGGGTGCGGTAACCACAC CGCTGCTGGGCCTTGTGCATCTGGATCATCAGGGCGCAGCCGGGCTGGAGATGGGTTACAACCAATGGTT ATCGGGTAAAGCAGGCAGTGAAAAGGTGCTGGTGGACGGGCAGGGCGAGGTGCTTCATGTTGTGGGTGCG CGGCAGACGCCAGTGCCCGGCCGCGACCTGCATTTGACTATCAACCCGCAGATTCAGTATTGGGCCTACA TGACGTTGTTGGCAGCGCAACAGCACTTTGGCGCCACAGAGGGCTCGGCGGTGGTAATGAATGCGCAAAC CGGGCAGATTCTCGCCATGGCGAGCGTGCCCTCCTGTAATCCCAACGCGCGCGCCGGTTGCGGCGACCCT TCTGATTATGTGAATAATGCCGTGCATCAGGCCTTTGAGCCCGGTTCAGTCATGAAGCCGTTTATGGTGG CTGCGGCACTTGCAACGGACAGCATCCAGCCGGACCAGAGCTTCAATGTCTCCCGCTGCCTGCCCGTGGG CGGTTTTTGTATCCGCGACGATGTGGTACACCATGAATTGAACGTGGCGCATATCCTCAAGTACTCCAGC GACATTGGTGCGGCTAAAATTGCTTTGCGGACGCCCGCAAAGGCTATTTATGACATGTACCTGGCGGCAG GTTACGGTCAGGAGCCGAATCTCGGTTTTGCGGGTGCTACGGGTGGCGTGCTTCCCCAGCCCCAGACCTG GGATAAGGCGCGCCATGCCACGATTGCGCTGGGCTACGGGGTGTCCGTGACGACCCTGCAATTGGCAGAG GGTTATGCCGCCATCGCCAACGGAGGCTATCATGTTACCCCGACCTTGATTGCCGGACAGTCGGTGCAGC GCGTGCGGATCATGTCCGCGGGCATCGCTGCCCACCTGCGGCACTGGCTAGAGGGGGTTTGTGCGGCCAA TGGCACCGGTATCCTCGCTGCCATTCCCGGCTACGCGGTGGCCGGTAAAACGGGAACTGCGAATATGGCC AATGGCAAGGACGGCTTTTTGAAGAACAAGACCAATGCCACTTTTGTCGGCTTTGCACCGGGCTTTGCGC CGAAGCTGGTGATGGCGGTGACTCTGCGCGGGAGTACTCGCTACTGGAATTTCGGTGGGGTCGAGTCAGC ACCGGTGTTCCGGGTAACCATGCGCCATGCCCTTGAAGAGTTGAACATTGCGCCACGCTGGTGCGGAGGT AAGGCTTGTGCCTCTAAGCATAATCACATTACGACCACTCAGGCCGAAATCTGGGCGGAGGGAGGCGGCA AATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 279853-281607	Sequence	Acife_0261 - penicillin-binding protein transpeptidase
ORF... 281604-281915	Sequence	Acife_0262 - hypothetical protein