Sequence retrieval

Genome: Acidithiobacillus ferrivorans SS3
Start position: 190862
End position: 192442
Length: 1581

DNA sequence
>NC_015942, from 190862 to 192442 (1581 bp); Acidithiobacillus ferrivorans SS3 TCAATTCAACGAGGCGAGGAGGTTCCGGCCTTTGTCGGTGATGCGGTAGCGCTGCTTGCTACTGGTCGGC TTGTCCGGAATGGTCATTTCCAGCAGGCCATCGGCAAGCATTGGCTTGATGACCTGATCCCGAAACTTGG TGCGGTTGGTCCGGTCAGCGACGGCCATGAGATCTGCAATTGGGCTATCGGCCAAGCACTTGTGCAAGAT TTCAACCTGGTCCCGACTTAGTCCCGACTTGATCCCGGCACCTGCCGCCGTATCGCCAGCGCCCATTTTT ACCGATGGAATCGCCGCAGTGCCGGTTGCTGACTCTGCTACCTCTAATGCCGCCGGGTGGATCGGTAGCC GCACCATGAAATAGCTGTGGTCTTCGTCGAACTCGAATTCGGCAGGCGGGGAGCCATTCTTTTCCATGGC TTCCATGATCTTGGGGATGCCAGTGGAACGGCCTTCGGTGAATTCCAACTCCTTCAAAAATTCACCGATG CGGCGGTTGCGATAGCGTCGTGGCCGGGCGCGCCCGGCGCGCAGTTGCTCCAGCCGCACCGAGCGATCCG GCCCAGGGTAGCTGATGACCATCAGCTCTGTGTGTTCGAGACGGATTTCGATGGGCTCGCGCGTGTCGTA GCCCCGGTGATAGACCGCATTGACGACGGCTTCTTCGAGGGCGTCGTAAGGGAAGTTTTCGACGCGGGTT GCTTCGGCTCGTCCGTGATGCTTGATGACGGTTTCGGTAATCAGGTTGCGCTTGATGTAATCGAGCGCGT CGCGGGTCATGCGGGGTATCGGGCCTTTGAATATTTTTTCCGAGAATTTATTTCCACCCGGCCCTTCCTT GGGAAACCAGACGACATCAATCTGCATGACAGGGAAGAAGCGCCATGGCTCCGGATTAAACATCATCAGG CCGACGTTGAGGGGGAACGGCGCTTCGTCTGTCCCACCCACGACGCCCATCTGGCGGCCAAGTTCTACCA ATGAAAGATCGGGTGCCTGCTTGGCCAGATCACTGCCCACTTGTTGCAGATAGTCTTGCATCAGTTCACG GGAGAGGCTTTCCACATTGGCCTGCTGGTTGACGCGATCGTCGTGGGGGATGGTGGCCGCCAATGACATC AGCTCAGTTTCGTCTGCCCCCTTGGCGCGTATGGTGCTGGAGTTTTTGCGGATGTAGTAGGCGTAGTCTC TGCTCTCTTTGCCCAGACTGATTTTCGCTTTGTAGGGACGCGTCGGCCCACCCAAAGCCCAGAGCACCAG AACGTGCCGCCCCTCGATTTCCACGGGAACGGCAATAGGGTGGTAATAGGGGGCAATGGCATTGAAGCCG AGGTTTAGCAGTTCCTTCTGGATGGCGTCGATTTGCGCGGGGTCGAGGCCGACGGGCGGCAGTACGGGAC GACCGTTTTCTTCCTGAATGCCGATCAGGATATAGCCACCGCCCAGATTGTGAATGTCGTTGGCGAAAGC ACAAAGCGTGTGGAGGACAGCCTCCGGGTTCCAGCCAGCCTTGAACTCCAGGCGTTCCCATTCGACGGGT TTACCGTGGAGGAGGTCGTTGATGTTGATGGGTAACGGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 190862-192442	Sequence	Acife_0178 - putative transcriptional regulator (Protein is coded in complementary strand; from 192442 to 190862)