Sequence retrieval

Genome: Acidithiobacillus ferrivorans SS3
Start position: 1551444
End position: 1552703
Length: 1260

DNA sequence
>NC_015942, from 1551444 to 1552703 (1260 bp); Acidithiobacillus ferrivorans SS3 TCACCCCCCATGCGTCACCACGGCGCCCATCTTGAAGATCGGCAAATACATGGCGACCACCAGGGTGCCG ACGATGATGCCGAGCACCACCATGATCATCGGCTCCATGAGGGTGGAGAGGCGGTCTACCGATTCTTCCA CGTCCGCTTCGTAAAAGTCGGCGACCTTGCTGGACATGGTTTCGATGGCGCCGGTTTCTTCGCCGATGGC CAGCATCTGCGTGGCCATGATGGGGAAGACGCCGTCGGCTTTGAGCCGGGTGGTGATGCGACCACCACCG GCGACGTCGTCACGGGCCGCCAGGACGCTGCGCTCGATAATGACATTGCCCGAGACCTTGGAGAGCGTTC CCAGTGCTTCCATAATGGGCACGCCCGCCGCCTGCATGGTGGACAGGGTGCGCATAAAGCGCGCCACGGC GCCTTTAAGCAGGATGTCGCCTAGCACCGGGATTTTCAGGGAGAAACGGTCTACCACGGTCTTGAAAGAC AGGTTGTGCTTGTAGGCGTAGACAAAGAGCCAGACCGCGGCGATGGGCACGAGCACGACAATATACCAGT GACCTTTCATCCAGTTGGAAATATTGACGACAACCTGCGTTAACAACGGCAATGTAGCGCCGAAACTGCT GAAGAGCTGCGAGAATACCGGGATGACGAAAATCATCAGGATGACCACGACCACGACCATGACGGTGATG ATGGCCGCCGGATAGAACATGGCCGACTTCACCTTTTTATTCAGTGCCAGGGTCTTTTCGCGGTACTCGG CCAGACGCAACAGAATGGTGTCGAGGATGCCGCCCTGCTCACCGGCGGCGATCAGGGACACAAAGAGGCG GTCGAAGTACTTGGGAAAGTGGGCCATGGCTTGCGACAGGGATTCGCCTTCCTGGAGATGTTGGAGAATG CCTTTGAGCAATTTTTTCATACCCACGCCGGAAGTGGCGGAGATGAGCAGCTCAAAGGACTGCACGATGG GCACGCCGGCGTTGATCATGGTGGAGAGCTGGCGGACCATCACCACTAACTGCCCTTCTTTGACGCCTTT TTCCCCGAACAGGGGTTGCGGCACTTTGCGCACAGCGGTGGGCATCAGCCCCGAGTGGCGTAGATTGGAG CGCACGATATTGGCGGAGACGGCGTTCATTTCGCCTTTTTTCTTTTCTTTCCCGTCGGGAGTGCTCGCCT CCCAGGTAAAGTCGTAGGTCTTTGCCGCGCCGATTGCGGATTTCTTGTCTTTATTCAGGATTGATGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1550978-1551454	Sequence	Acife_1552 - type IV pilus modification protein PilV (Protein is coded in complementary strand; from 1551454 to 1550978)
ORF.. 1551444-1552703	Sequence	Acife_1553 - type II secretion system F domain-containing protein (Protein is coded in complementary strand; from 1552703 to 1551444)