Sequence retrieval

Genome: Acidithiobacillus ferrivorans SS3
Start position: 1010949
End position: 1012469
Length: 1521

DNA sequence
>NC_015942, from 1010949 to 1012469 (1521 bp); Acidithiobacillus ferrivorans SS3 GTGAAGGGTGGTGCACTGAAAAAACCGCACTTTCTTGTGCGGCTTTTAGCCGCGTTGATTTTGGGTGCAG CCTGGCCCTTGGCCTTCGCGCCCTTGGCCTTGTGGCCGCTGGCCATCGCCCTGCTTTTTGGCCTGTTTTG GCTGGCGAGTACAGAGGAACGTCCGCGCCGTGCGGCCGCTCTCGGGGCCGTTTTCGGTTTTGCGGCCTTT GCGGCGGGAACCAGCTGGATCGCCATCACCCTGCATAATTTTGCCAACATGGATTGGGCATTAGCTGAAG GGGCCGTAGCTTTGCTAGCGGCTTGTTGTGCCGTCTATCCGGCGCTGGCCTTATGGCTGGCCACCCGCTT TTTTGTCGGCAATGCCCGGTTACTGGCCCTGCCGGTTCTGTGGATTTTGGGGGAGTGGGCGCGGGCACGT CTTTTTACAGGCTTCCCCATGTTGGCGACGGGGTACACCCAGACCCATGCCTTCCTTGGCGGCTTTGCAT CCTGGCTTGGCCAGTACGGGGTGGGGCTGGCAACCGCCGGGGCGGCGGCTATTCTGGTTTATATGCTGCA ACGGCGGCAGTCCCGCCAAGTGTTGATGGGGGGCGTCGCGGCGCTCCTCGTGATTTCCGGCGTAGCCATG GCGGCGGCATCCGTGTCGTTTACCCATCCCGCAGGCAAACTGCTGCGGGTGAGTCTGCTGCAGGGGAATG TTGCCATCACCGAAAAGTGGAATGCGGGTAAAATCAGCGACATTTTGCATCACTACGTTGATCTGATCTT GCAGGTACCGCCGAACACCCAATTGATCGTTTTGCCGGAAACAGCCTTTCCTGTTTTTCAGACGGAAATT CCGGGCCTGATCGCGCAATTGCAGCAGTGGTCAGCGATGCACCATAAAATTCTGCTCATCGGTGTCCCCG AAGATGCGGGCCACAAATATTACAATGCGGCCATGGAAATCAATGGTAAGGCGCCTTTGCGCTGGTATCG GAAAGAGCATCTGGTGCCTTTCGGGGAATACATCCCGATGCCGAGCCTGCTCACGTCGCTGGTGCATCAT TTTTTACCGGGACTGGGGAGCTTTTCTTCCGGGCATGGCCCTTATGTTTTGCCCGTGGATGGACAGAAAG CCGGTATAACCATCTGTTATGAAGAGTCCTTCTCCCGGGATGTGCGCAAGGGTGTAGAAGAAGGTGCCAC CTTTCTTCTGAATATCAGTGATTATGCCTGGTACGGCCATAGCATCGCCGCAGCGCAGAGTCTGCAAATG GCGGCGATGCAGTCCTTGCAGGAGCAGAAACCCGATATTCGGGCGACCAACACCGGAATCACGGCCCTGA TTTCGCCACGCGGGCAGGTGCGTGCACAATTACCCCAGTTTGTGCTGGGCACCCTGAATGAGGCCATACA GCCGATGGCGGGGGAAACGCCTTACGGGCGCTGGGGCAGTCTCCCTTATCTGTTGTTGAGCTTGATTCTG CTGCTGATGGCCGTCGTCCTCAGTCGCCAGCAATCATCATTTCGTCAATAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 1010068-1010952	Sequence	Acife_0985 - CBS domain-containing protein
ORF.. 1010949-1012469	Sequence	Acife_0986 - Apolipoprotein N-acyltransferase
ORF... 1012438-1013784	Sequence	Acife_0987 - peptidase U62 modulator of DNA gyrase (Protein is coded in complementary strand; from 1013784 to 1012438)