Sequence retrieval

Genome: Ramlibacter tataouinensis TTB310
Start position: 454592
End position: 457651
Length: 3060

DNA sequence
>NC_015677, from 454592 to 457651 (3060 bp); Ramlibacter tataouinensis TTB310 TCAGCCCGCCAGCAGGGCGACGTTGCCGCCGGCCGCCGCCGTGTTCACGGTCAGCGTGCGCTCGGCGCAA AAGCGCGGCAGGTAATGCGGGCCGCCGGCCTTGGGCCCGGTGCCCGACAGGCCCTCGCCGCCGAACGGCT GCACGCCCACCACGGCGCCGATCATGTTGCGGTTGACGTAGACGTTGCCCACCCGCGCCCGCGCGGCCAG CGCCTGGGCGCGGCTGTCGATGCGCGTCTGGATGCCCAGCGTCAGGCCGTAGCCCAGCGCGTTGACCTGC TCGACCACCGCCTGCGGGTCGCCCGACCAGCGAGCTATGTGCAGCACCGGGCCGAAGATCTCGCGCTGCA CGTCGGTGACGCGCTCCACCTCGAAGGCCTGGGGCGCGATGCAGTGCGGCGGCGCGGCGGGCGCCTCGGC GGGGGCAAGCACCGCGCGGGCCGTCTGGCGCAGCCGCTGCAGTTCCCCCTGGAGGCCGGCGAAGGCCTGC GCGTCGATCACCGGGCCGACGTCGGTGGACAGCAGGGCCGGGTCGCCCACCGTGAGCTCGCGCGCGGCGC CGGCCACCATCTCGATCACCGCGTCGGCGATTCCCTCGTGCACGCACAAGAGCCGCAGCGCCGAGCAGCG CTGGCCGGCCGAGCGGAAGGCGCTTTGCACCACCGCGTCGGCCACCTGCTCCGGCAGGGCGGTGGAGTCG ACCAGCATGGCGTTGATGCCGCCGGTCTCGGCGATCAGCGGGACGATGGCGCCGTCCTTGGCGGCCAGGG CGCGCTGGATCAGCCTGGCGACCTGGGTGGAGCCGGTGAACACCACGCCGGCGACACCGGGCGCCGCGAC CAGGGCCGCGCCCACCGTCTCGCCGGGGCCGTGCAGCAGCTGCAGCGCGTCCGGCGGCACGCCCGCCTCG TGCAGCAGGCGCACCGCTTCCCGGGCGACGGCCGGCGTCTGTTCGGCCGGCTTGGCCAGCACGGTGTTGC CGGCGGCCAGGGCGGCCGCGACCTGGCCGGTGAAGATGGCCAGCGGGAAGTTCCAGGGGCTGATGCAGAC CCAGGGTCCGCGGCCGGCCAGGCGCAGTTCGTTGCGCTCGCCGGTCGGCCCCGGCAGCAGCGCGGGCTGC ATCACGCGTTCGGCCTCGCCGGCGTAGTAGCGCAGGAAGTCCACCGCCTCGCGCACCTCGGCCACGGCGT CGCCCCAGGTCTTGCGCCCCTCGGCGACCAGCAGCGCGCACAGCGGCGCCATGCGCGCCTCCATGGCATC GGCGGCGCGGCGCAGGGCTTGGGCCCGTTCGGCCACGGGCCGGGCATCCCAGGCCGGGAAGGCCGCGGCG GCGCGCGCCACCGCGGCCGGCGCTTCGCCCGCCTGCGCCTGCGCCACCGGCGGCACCCGCAGCTGCGCAT GCGCCGCCAGCAGCGGCGCGCGCATCGCCGCCACCGCCAGGTCCAGGCCCGCGCTGTTGCGCCGGCCGGG CCCGTACAGCCCGGCCGGCAGCGGCAGGGCCGGCTGCTGCGCCAGCCGCGCCAGCGAGGCCCGGGCCTGG GCCAGCGGCGAGGCCAGCAGCTGGTCCAGGCCCACGGATTCGTCGGCCAGCTGGTGCACGAAGGAGGAGT TGGCGCCGTTCTCCAGCAGTCGGCGCACCAGGTAGGCCAGCAGGTCGCGGTGCTTGCCGACGGGTGCGTA GACCCGCACGCGCGGCTCAAAGCCTCCCCCGGATGGGGGAGGTTGGGTGGGGGCGAGCCCAGCCTGCCCC GAAGGGGTGTATCGCGAAAGAATTTCGCGATACACCCCCTCTCCCATGCCGTGCAGCCGTTGCAGTTCGA ATGCGGCACCCTCGCGCTCGGCCATCTGCCGGATGGCGGCGATCGTCCCGGCGTTGTGCGTGGCGAACTG CGGGTAGATGGCGTCGGCGGCCTGCAGCAGCGCCCGGGCGCAGGCCAGGTAGGACACGTCGGTGTGCTGC TTGTGCGTGAACACGGGGTAGTGCGGCAGGCCCAGCTCCTGGGCGCGCTTGATCTCGGCGTCCCAGTACG CGCCCTTGACCAGCCGGCACATGAAGCGCAGGCCCAGGCGCCGCGCCAGGTCCGCCACGTGCTCGATCAG TTCCAGCGCGCGCGTCTGGTAGGACTGCAGCGCCAGGCCGAAGCCGCTCCAGCGCGGATGCCGCTGCGCG ACGCTTTCGGCCAGCGCCTCGAACACCGCCAGCGACAGCTCCAGCCGGTCCACCTCCTCGGCGTCGATGG TGAGGTTGAGGTTGGACCGGGCGGCCTGCTCGCACAGCTGCCACACGCGAGGCACCAGCTCGCGCAGCAC GCGCTCGCGCTGCGCCTCCTCGTAGCGCGGGTGCAGCGCGCTCAGCTTGATGGAGATGCCGTCGCTCTCG GCCGGCGCCCGCGCGGCGTCGCCGCGCGCCGCGATGGCGGCGATGGCCGCGCGGTAGCTGGCCAGGTAGC GCTCGGCGTCGTGCGCGGTGCGCGCGCCTTCGCCCAGCATGTCGTAGGAGAACAGCAGCGCGGGCTGGGC GCGCCGGGCTGCATCCGCTTCGTCCAGCGCGGCGCCGATGTCCTGCCCCAGCACGAACTGGCGGCCCAGC AGCTGCACCGCGCGCAGCGTCGCGGCCACCACGGTGCGCGCGCCCAAGCGGGCCAGCAGGCCGCCGCCGT CCGGGGCGTCCGCCTGCTCCGGCAGCAGCTTCTTGGACAAGGCGATCGCGGCATTCGACAGCCGCGCCAG GGCGCCGTCGGCCGCGCCGGAGAAATCGGCACGGCCCAGCTGGTCGGCGGTCAGCGCCACGGCAGTCTCG GCGTCCGGCACGCGCAGCAGGGCCTCGGCCAGGCGCATCAGCGCCAGCCCCTCGGCGCTGGAAATGGGGT ACTCCTTGAGCAGGCTTTCCATCGCCCAGAAAGGCGGCGGATGCCGGCGCACCGCCTGCACCCAGGGGGC GGCGGCGGCCGCGGCGGCAGCCCAGTCGAGGGCGCCTTCGAGCCGGCGCAGGCGGTCGGCCAGGACGGCG GCTTCGGGGCGGTAGGGGGAAGGCAGGCGGTCGGCGGCAGGTGTGTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 454592-457651	Sequence	Rta_04440 putA bifunctional putA protein : proline dehydrogenase, delta-1-pyrroline-5-carboxylate dehydrogenase (Protein is coded in complementary strand; from 457651 to 454592)