Sequence retrieval

Genome: Sphingobium chlorophenolicum L-1
Start position: 257993
End position: 262168
Length: 4176

DNA sequence
>NC_015593, from 257993 to 262168 (4176 bp); Sphingobium chlorophenolicum L-1 ATGGCGGAGGAACTGCCCGCCACCCCGCCGCGCCGCCGCCGCGCATGGGACAGCCGCTGGCAACGCTGGG TCGCGGGCATCATCGCCGCGCTGCTGCTGATCGTCGCGGGCGCCCTGGCCTGGCTCGACACCGCGCCCGG CCACCGTTTCCTCGTCGCCCGCATCGCCCGGATCAGTCCGCCTTCGGGCCTGCGCATCCATGTCGACCGG ATCGAGGGCAGCATCTACCGCAAGGCGATCCTCCACGGGCTTACCCTGTCAGACCCCAAGGGCGCCTTTT TCGACGCGCCCAGGGTGGAACTGGACTGGTGGCCCTTCGCCTGGGCTTACAACCGGCTGGACATCGACCG GCTCGTCATCCCGCAGGCGACCCTGCGCAAGCTGCCGAAACTCAACCCCAGCGAGCGGCAGGGGCCGATC CTTCCCGGTTTCGACATCCGCCTGATGCAATTTTCCGTCGGCCGCCTGACCATCGACCGGGGCGTCAACG GCAAGGTGCAGGAGGCCACCCTGTCCGGCGATGCCGACATTCGCGGCGGCCGGGCGATCATCGACCTGTC GGCCCGCGTGCTGAATGGCGAGGATGCGCTCAACATCGCGCTCGACAGCCGCCCGGACGACGACAGGTTC GACCTGGACGTCACCGTCAACGCGCCCAAGGGCGGCGTGCTGGCGGTGATGGCGGGCTTGCGCCAGGATG CCAATCTGCGCATCCAGGGCAAGGGAAGCTGGACCCGCTGGGACGGCCGCCTGGCAGGCACGCTCGACAG CGAACCCGCCATCGACCTGAAACTCGCCGCCCGCAGCGGAGCCTATACCGCCACCGGTACGATCGAAGGG TCCGCCATAGCGGGTCAGGGCCTGTTCCAGCGCCTCTCCGCCCCGCGCCTCGCCATCCAGGCCAGCGGAT CGATGACTGACCGCGTGATCGACGGCAAGCTCTCGCTCCGCTCCGCCGCCATCGACCTCAGCGCCGACGG CGGCATCGACCTGCGCAACAATGCGCTCGACAATCTGCTGGTCGATGTGAAGCTGGCCCGGCCGCAAGCC CTGCTCAAGTCGATGAACGGTCGCGACATCGCGGCCCGCGTCCGTCTGGACGGCCCGCTCGGCGCTTTCG GCTATGAATATCTGCTGACGGCAAAGCAACTGGTCTTCGGCAAGACGGTGATCCACGACGTCCGCGCCGA CGGCAAGGGGCGCAAGGCGAAGGGCGGTCCCGCGCTGATCCCGGTCCATCTGCGCGCCCGCCGCCTGGAC GGGCAGGGCGAACTGGTCGAGGGCATCATCCGCAATTTCGTCCTGGACGGCGTGTTGCAGCTCAGGAACC AGCAGATCGTCAGCAACCCGATGACGGTCCGATCCGACAAGCTGCGCGGCAAGCTGATCATGGTCGCGGA CCTGAAGACGGGTCGCTATGATTTCGGTCTGGACGGGCAGATCAACGGCCTCGCCATTCGCGGCCTCGGC ATCGTCGACCTGACGTCGAAGCTGCGCGCCATCCCCGGTCCCAGGGGCGCCTTCACGCTCAGCGGCAATG CGATGGCGCGGATGCGGCGGCTGGACAACAGCTTCCTGCGCTCGCTGGGCGGCGGCCTGCCCGTGTTGCG CAGCGGCCTGACGCTGGGACCGGACGGCCAGTTGCAATTCACCAACCTCCATCTCCAAGCGCCGCTGATC ACCCTCGCGGCCAAGGGCTATCGCCGCCGCGACGGCAGCTTCCATTTCGAGGGCGGCGGCCAGCATAAAA GCTATGGCCCCTTGCGCCTGACCCTGGACGGCAAGATCGACCGCCCGACCGTCGACCTGGTCCTGCAAAG CCCGTTGAAGGCGCTTGGCGTCACCGACATGCACGCCCATCTCGTCCCCGACGCGGCGGGCTACAGCTTC ACGGCGGAGGGGGGATCGACCCTTGGCCCCTTCACCGGCAATGGCGCGGTCCTGCTGCCGCCGCGTGGCC AGGCCATCGTCCGCATCGCCCGCGTCGCGGCGTCGGGCGTCACCGCCAGCGGCGACCTGCGCCCGGTCAC TGGCGGCCTCGACGGCACGCTCAACGTCAGCGGCCCCGTCACCGGCACCATCGCCTTCGCGCCCGTCAAC GGCATCCAGCAACTCATCCTCAAGCTGGACGCCACCGACGCCAGCTTCGACGGCCCCACCAGCATCGCCA TCCGCCGGGGCAAGCTGGACGCGACGATCCTGCTCGATCCGTCCGGCACCTCGATCAACGCCACCGCGCA GGCGCGGGGCATGCGCGTCGGCGCGATCTCCATCGGCCGCCTCGCCGCCAATGCGGAACTGGTCGATGGC AGCGGCAAAGTCCGCGCCAGCATTTCCGGCCAGCGCGGTCGCCTGTTCGACATCACCGGCGAAGCGCAGG TCGAACCCGACCGCATCCAGCTTCAGGCGAGCGGCACCATCGACAAGCGCCCGATCAAGCTGACGCGTGC CGCCATCTTCACCCGCACGCAGGACGGCTGGCGGCTCGACCCCGCATCGATCAGCTTCGCGGGCGGATCG CTCCAGCTTGGCGGCGAACTCAGCGGCCAGACCACCAGCATAGAGGCGCGCCTGCAAAGGCTGCCGCTGT CGCTGCTGGACATCGGCTATGACAATCTGGGCCTGGGCGGCACCGCCACCGGATCGCTCAGCTATGTGCA CACGCGCGGCGAAATGCCTACGGGCAAGGCGGAACTGCGCGTGCGCGGCCTGTCGCGATCCGGCCTGTCG CTCAGCTCCCGCCCGGTCGATGCGGGGGTGAACGCTGTCCTGACCCATGACCGGCTCGCTACCCGCATGA TCTTCGTCGCGGACGGCCAGACCATCGGCCGCGCCCAGGCGCTGCTGACCCCGCTGGGCACCGAAGGCAG CCTGGTGGATCGGCTCAACCGCGCGCCCCTGTTCGCGCAGCTTCGCTACAACGGCACCGCCGACACGCTG TGGCGCCTGACCGGTGTGGAGATCGTCGACATATCCGGCCCGGTCGGCGTCACCGCCGATGCGCGGGGTA CGCTGGGCAACCCTGCTATCACCGGATCGCTGACGACCGACAATGCCGCGATCAACAGCCCCGTCACCGG CATGCGCCTGCACGGCGTCAAGGCGCGGGGCCGCTTTTCCGGCGCGCAACTGGTCATGACGGATTTCGCC GCCACCGCGCAAAATGGCGGCAGCGTCACCGGCACCGGCAGCTTCACCTTCATGGGGCTGGGGGGCGTCG GCATGGACCTGCGCCTTCAGGCTGAAAACGCCGCCTTGCTGGAGAGGGACGATATCGCCGCCACCGTCAC CGGCCCGATCACGCTCAATTCCAGCGGAAAGGGAGGCATCATCGGCGGCGACCTGATCCTCAACCGAAGC CGCTTCACCATGGGCCGCGCCGCCGCCGTCGCGCAAATCCCCGAATTGAAGGTGATAGAGATCAACCGGC GCGGCGACGAGATAGAGCGCCCCCGCGCAGTCGCCCCCTGGGATCTGGCGATCAAGGCCCGCGCCCGCAA CCGCCTCACCGTCACCGGCCTTGGCCTCGAAAGCGAATGGCGCGCCAATCTCGATATCGGCGGCACCGTC GCCAGCCCGGCCATAGCGGGCACCGCCGATCTGGTTCGCGGCACTTATGATTTCGCGGGCCGCCGTTTCG ACCTGAAGGAAGGCAAGATCCAGTTCGACGGCCGCACCCCGGTCAACCCGACGCTCGACATCGATGCGGA GGCGAATGTCAGCGACCTGACCGCCACCATCCATGTCGGCGGCACTGGCCTCAAACCCGCCATCACCTTC ACCAGCGTCCCGGCGCTGCCGCAGGATGAACTTCTGTCCCGCATCCTGTTCGGCACGTCCATCGTCAACC TGTCCGCGCCCGAAGCGCTGCAACTCGCTTCGGCGGTCGGCTCGCTCCAGGGACAGGGCGGCCTCGACCC GATCAACGCCGTGCGCAGGGCGGCGGGCCTCGACCGCCTGCGCATCATCGCCGCCGATCCGACGCAGGGG CAGGGGACGTCCATCGCGGCGGGCAAATATCTGACCCGCAAAACCTATGTGGAACTGATCACCGACGGGC AGGGCTATTCCGCCACCCGCATCGAATATCAGGTCACGCGCTGGCTCTCGCTGCTCGCCGCCATCTCCAC GCTTGGCCGTGAAAGCGCCAACGTCCGCGTTTCGAAGGATTATTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 255951-257993	Sequence	Sphch_0252 - surface antigen (D15)
ORF.. 257993-262168	Sequence	Sphch_0253 - hypothetical protein