Sequence retrieval

Genome: Arthrobacter phenanthrenivorans Sphe3
Start position: 520682
End position: 522091
Length: 1410

DNA sequence
>NC_015145, from 520682 to 522091 (1410 bp); Arthrobacter phenanthrenivorans Sphe3 TCAGTAGCGTGCCGCGTACGGGTAGAGCTGCATGCCGGCCATGGCGCTGAGTTGGGTGACGCCGATGCCC TCCTGCGGGTTCAGCGCCTGGACAACCTGCCCGTTCCCCAGGTAGATGGCCACATGGTAGAAGTTCGACG GCGATCCCCACACCAGCAGGTCGCCGCGCCGTGCCTGCGAGATGGGCACGTGGACCGGTGCGGCGGCGTA CTGTTGGGCAGCCGTGCGCGGAAGATACTTCCCGGCGGCCGCGAAGGCGGTCTGCACCAGCCCTGAGCAG TCAAAGCCGTAGGGGCCCGTTCCGCCCCACTGGTAATAGTAGGGAGCGCCCACCTTGCCCAGGGCCACCG AGATGGCCGCTTCGTGGGTCCCGCCGCCCAGCGGCGGAGCCGGTGCGGGCGCCGGAGCCGGAGCCGGTGC AGGCGCTGGCGCTGGCGCTGGGGCCGGTGCGGGCACCGGAGCTGGAGCTAGTGCGGGTGCCGGAGCGGGT GCTGGAACCGCAGGTGGTGCCGCAGGTGCCGGAGCCGGTGCAGGCGCAGGCACTGGCCCTGGGGCCGGAA CCGCCGGCGCGGCGGGTGCTGCAGGGGCGGGCGCTGCCCCCTCCGGACGGTCAACCGCCGGGGCACGCTG CTCCTGGGGTTGTGCTGCCGCTGCCCGCTCCGCTGCGGCCGTCAGCGCCGCCAGCCGGGCCTCTTCCCGC TGCCGGTCCAGGGCATCCACCCGGGCCGATTCCAGCGCCACGGTGGTGTTCCGCAGCTGTGCCAGCTGCT CCACCAGGACGCTGCGCTGGGTTTTGGCATCCGCCACGGCTTTGGCAGACGCAGCATTGGCTTCCTCGGC CTGGGTCTTCCGTGTTTCGGCGGTCCTGGCCGCGTCGTTGGCGGCCTTGGTGGCATCCTCTGCGGCTGCG GTCAGGGAGCGGGACGCCTCGGCTGCGGAGTCTGCCGCCTGGAAGGCCCGGTTCCGGCTGGCCGAGAGGG CCTGCAGCGTGGCCGCCTGCTGAAGGGTTTCGCTGCCGCCGCTGACAAAGGTGCCCAGGCTGGGGTTCAG TCCGCCGTTCCGGTACAGGTCGCCGGCAAGCTGCCCTACCTCCTTGCGCCTCTTGTCCTGCTGTTCCTGT GCCGTGGCGGCCCTGGCCGACGCAACGTCTGCAGCCGAAGTGCGCTGCTGGAGCTCCACCAAGGCTTCGC TGTACGCATTGTTGGCCTGCATGGCTACGGTAAAGGCGGCCTGCTGTGCTCCCGCTGCCTCGTCAAGGAT GCGTTCGATGGTGGTTACCTGGTCCGCCGCTGCAGCCTCGTTTGATTTGGCAGCGGCGATGTCCTCCGCG GAGGGGACTTCCGGCGAGGCGGGGACGGAAATGCCCGGGTATTGCGGCGCGGGGAAGGCAATTGCCGCGG TGGCTGGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 520682-522091	Sequence	Asphe3_04990 - cell wall-associated hydrolase, invasion-associated protein (Protein is coded in complementary strand; from 522091 to 520682)