DNA sequence >NC_015145, from 415537 to 415690 (154 bp); Arthrobacter phenanthrenivorans Sphe3 TAGCCTCCAAAGCCTAGCCTCCAAAGCCTAGCCTCCAAAGCCTAGCCTCCAAAGCCTAGCCTCCAAAGCC TAGCCTCCAAAGCCTAGCCTCCAAAGCCTAGCCTCCAAAGCCTAGCCTCCAAAGCCTAGCCTCCAAAGCC TAGCCTCCAAAGCC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI