Sequence retrieval

Genome: Arthrobacter phenanthrenivorans Sphe3
Start position: 26231
End position: 27628
Length: 1398

DNA sequence
>NC_015145, from 26230 to 27627 (1398 bp); Arthrobacter phenanthrenivorans Sphe3 TCACGAATGCTTCACCGCCTCGGTAGGAGTGTCAATGCCCATGGATTCTTCTGCTTCGCCTGGCCGCAAT GGCGTTGTGTCCACCGGGCCGCGGGCCGTGTGCGAGATCATCAGGAGCAGGCCAACAATGATCCAGTTGG CGAGCAGCGAAGAGCCGCCGGCCGCCAGGAACGGCGTGGTCAGGCCTGTCAACGGGATGAGCCGCGTGAC GCCGCCGATCACCACGAAACACTGCAGTGCCACAGCGAAGGACAACCCGCAGGCCAAGAGCTTGCCGAAC GCGTCGCGGGTGCCCAGGGCAGCGCGGAAACCACGGGTAAAGAGCAGCAGGTACATCATGACGACGGCGA AGAGGCCCACCAGGCCGAGTTCCTCGCCGAGGGACGCGATGATCATGTCGCTGTTGGCGAAAGGAACGAG GTCCGGCCTGCCCTGGCCTAGTCCCGTGCCCACCAGGCCCCCGTTGGCCATGCCGAAGAGGCCCTGGACG ATCTGTCCGCTGCCGCCGGGTGAGCGGTCGTAGACCTCGGGGGTGAAGGCATTGAGCCAGCCGTCGATGC GGAGTTCCACGTGGGAGAAGACCTGCGCCGCAACGAACCCGCCGCCAAAGATGAGGGCCAGGCCGATCAC CACCCAGCTGATGCGGCTCGTGGCGACGTAGATCATCACGATGAACAGCCCGAAGAACAGTACCGAGGAG CCGAGGTCGCGCTGGAAAATCAGCACGCCGATACTGACGAGCCAGGCCGTGATCATGGGGCCCATGTCTT TGAAGCGTGGGAACTGCAGCGGCCCGATCTTGCGGCCCGCCAGCAGGATCAGGTCGCGGTTGGAGGAAAG ATACCCGGCAAAGAATATGGCGAGGGTAATCTTGGCAACCTCACCAGGCTGGAAGGTCATGGGACCGAGG CGGATCCAGACGCGGGCGCCCAGGATTTCGCCGGCGCTTATGCCAGGAATCAGCGGCAGGATGAGCAGCA AGGCACTGGCCGCCAGGGAAATAAAGGTGAACCTGCGGAGGATCCGGTGGTCCTTGAGGAACCAGATGAC GGCGATTGCCACTGCCATGGCGATCAGGGTCCAACGGAGCTGGTTGTTGCCGGTGTCGTCGCCCGGGGCA TCCAAGCGGTGGATCATCGCAAGCCCGAGGCCGTTAAGGGCAACGACCAGGGGAAGTATTACGGGGTCGG CATATTTCGCCCGGAAACGGAGGACACCGTGGAACACCAAGGCCGCTACTGCCAGAAGGCTGGACTGGAA CCAGAAGTCTGAATCAAACGCCTTTTCCTGGTCCACTCCCACCAGCATGTTGGCTCCGATGCCCACGGCC AGGGCCAGCAGGAGCAACGCAAGTTCAACGTTGCGCCGAGGTTTGGGGGTAGTGCTGAGTTGGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 24774-26234	Sequence	Asphe3_00250 - cell elongation-specific peptidoglycan D,D-transpeptidase (Protein is coded in complementary strand; from 26234 to 24774)
ORF.. 26231-27628	Sequence	Asphe3_00260 - cell elongation-specific peptidoglycan biosynthesis regulator RodA (Protein is coded in complementary strand; from 27628 to 26231)
ORF... 27625-29259	Sequence	Asphe3_00270 - serine/threonine protein phosphatase (Protein is coded in complementary strand; from 29259 to 27625)