Sequence retrieval

Genome: Alicycliphilus denitrificans BC
Start position: 447470
End position: 449236
Length: 1767

DNA sequence
>NC_014910, from 447470 to 449236 (1767 bp); Alicycliphilus denitrificans BC ATGACCTTCTCCATCCACGGCCTGGCGATCGCACGCGGCATTGCCATAGGGCGAGCGGTGCTGGCAGCGT CCGGCAGCATGGAGGTCGCGCACTATTTCATCGAGCCGGAGCAGGTGCAGGCCGAGATCGCGCGCGTGCG CGGTGGCCGCAATGCCGTGATCGAGGAACTGCAGCGCCTGCAGGCGGACATGCCGCCCGACGCGCCGCCC GAGCTGGCTGCGCTGCTGGACGTACATCTCATGCTGCTGCAGGACGAGATGCTGGTCAGCGGCGTCAAGC ACTGGATCACCGACCGCCTCTACAACGCCGAATGGGCGCTGACCACGCAGCTGGAGATCATCAGCCGCCA GTTCGACGAGATGGAGGACGAATACCTGCGCGAGCGCAAGGCCGACCTCGCCCAAGTGGTGGAGCGCATC CTGCGCCGCATGAAGGGGGTGGCCGGCCCCATGGCGCCGCCGCCCAGCCGGGCGCGCCGCCAGGCCGAGG CCGGCGCCGAGGCCGAGTACCTGCAGGGCGACGCGGCCGACGTGCCGCTGGTGCTGGTGGCCCACGACCT GTCGCCGGCCGACATGCTGCAGTTCAAGCAGAGCGTGTTCGCCGGCTTCGTCACCGATGTGGGCGGCAAG ACCAGCCACACGGCCATCGTGGCGCGCAGCATGGACATTCCCGCCGTCGTGGGTGCGCGCCTGGCCAGCC AGCTGGTGCGCCAGGACGATTGGATCATCATCGACGGTGACGCCGGCACGGTGATCGTCGATCCCACGCC CATCATCCTGGCCGAATACGGCTTCCGCCAGCGCCAGCTCGCGCTGGAGCGCGAGCGCCTGACGCGGCTG CGCCATACGCCGGCCGTCACGCTGGACGCCCAGGCCATCGAGCTGCTGGCCAACATTGAGCAGCCCGGCG ACGCCGCCGCCGCTCTGCGTGCGGGCGCCGTGGGTGTGGGCCTGTTCCGCAGCGAATTCCTGTTCATGGG ACGCGGCGGCCGCCTGCCCGACGAGGATGAGCAGTACCTCGCCTACCGCGAGGCCGTGCAGGGCATGGAA GGCCTGCCCGTGACCATACGCACGGTGGACGTGGGCGCGGACAAGCCGCTGGACAAGGGCTACAAGGACC GCTCGCTCAACCCCGCGCTGGGCCTGCGCGCCATCCGCTGGAGTCTGTCCGACCCGGCCATGTTCCGCAC CCAGCTGCGCGCCATGCTGCGCGCGGCCGCGCACGGGCCGGTGCGGCTGTTGTTCCCCATGCTTGCGCAC GTGAGCGAGATCCGCCAGACGCTGGCCCAGGTGGATCTGGCGCGCGCCGAGCTCGATGCGCGCGGCGCCG TCTACGGCCCCGTGCAGCTGGGCGCCATGATCGAGGTGCCGGCTGCGGCCCTCATCGTGCAGCAGTTCCT GCGCTACTTCGACTTCCTCTCGCTGGGCACCAACGACCTGATCCAGTACACGCTGGCCATCGACCGCGCC GACGAGGCCGTGGCGCACCTGTACGAGCCGCTGCACCCGGCGGTGCTGCGGCTCGTGGCCGACGTGATCG CCGAGGGCGCGCGCCAGGGCAAGAGCGTGAGCGTGTGCGGCGAGATGGCGGGCGACGTGGGCATGACGCG GCTGCTGCTGGGCCTGGGCCTGCGCAGCTTCTCCATGCAGCCGGCGCAGATCCTGGCCGTCAAGCAGGAG GTGCTGCGCGTCGACGCGCGCAAGCTCGCCGACTGGGCGCTGCAGGTGCTGGCCTCGGACGACCCGGCCG CCATGCTGGCCCAGTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 447470-449236	Sequence	Alide_0428 - phosphoenolpyruvate-protein phosphotransferase