Sequence retrieval

Genome: Alicycliphilus denitrificans BC
Start position: 4083665
End position: 4084411
Length: 747

DNA sequence
>NC_014910, from 4083665 to 4084411 (747 bp); Alicycliphilus denitrificans BC TCACGCAAGCAGCAGCCAACCGAACCACGCGCCGGCGGCCAGCAGCAGCATGCTCACGAAGCGCTGCAGG CGGTGCCGGCCCAGCCCCTGCACGAGCCGCTGGCCCGCGAGCGAGCCGGCCAGCATCACCACGCCGCAGG CCAGCGCCAGCCCCCAGTCCAGCCGGTGCGCGGCGGCGTGCACGGCGCTGCTGGTCAGGGCCACGGGCAG CTGTATGGCCTGCGCTGCGGCCACGGCGAACTGCACCGGCTGGCGCCGCAGCATCAGCAGCGGCAGCAGC AGCACCGGCCCGCCCGTGCCTGTGAGTGCCGAGCCCACGCCCACCAGCAGCCCCAAGCCGGCCAGCGCGG GCCTGGGTAGCTGCGGCGCGGCCTCGGCGCCGGGCCCGCGCGCCGCAAGGCCGCGCCAGCCCGCGAACAG CACGAGCGCGGTCACGCCCGCGAGCAGCGCGCGCGCCGGCAGCGCCTGCACCAGCAGCGCGCCGCCCACC GCGCCAGTCAGCGCCCCCAGCAGCAGCGGCGCGGTGCGCCGGGCCAGGGCCGGGTCGCGCCGCAGCGGGC GCAGCGCCACCAGCGCGGGCAGCGCGAACGCGAGCGAGGCCGCCGCGATGGCCTGCGCGGGCTCCACTCC GCCAAAGCGCGTGAGCACGGGCACGAGCAGCACGCCGCCGATGCCCGTGGCGCCGATCAGCGCGCCGGCC AGCAGCACGGCGGGCAGGAGAATCCAGGCGTGGGCCAGCAGGTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4083665-4084411	Sequence	Alide_3909 - hypothetical protein (Protein is coded in complementary strand; from 4084411 to 4083665)