Sequence retrieval

Genome: Alicycliphilus denitrificans BC
Start position: 2455711
End position: 2458854
Length: 3144

DNA sequence
>NC_014910, from 2455711 to 2458854 (3144 bp); Alicycliphilus denitrificans BC ATGGCGCAGTTCTTCATCAACCGCCCCGTCTTCGCGTGGGTGCTGTCCATCGTCATCATGCTCGCGGGCG GGCTGTCCATCTTCACGCTGCCGCTGGAGATGTACCCCGAGATCGCGCCGCCGCGCGTGACCCTCAACAC CACCTACACCGGCGCCTCGGCCGAGACGGTGGAGAACTCCGTCACCCAGGTGATCGAGCAGCAGCTCAAG GGCCTGGACAGGCTGCTCTACATGAGCTCCACGAGCGACTCGGCGGGCCGCTCGCGCACCACGCTCACCT TCGCGCCCGGCGCCAACATCGACGTGGCCCAGGTGCAGGCGCAGAACAAGCTGCAGGGCGCGATGAACCG CCTGCCCGACGCCGTGAAGAGCCGCGGCGTTTTCGTGAACAAGGGCGGGCAGGACTACCTCGTGACCTAC GTGTTCACCTCGCCCGACCCGAACGTGTCGCAGGTGACCATAGGCGACTACCTGACCAGCAACGTGGTGG ACGTGATCGCGCGCGTGGACGGCGTGGGCGACGTGACCGTGTTCGGCACCAACTACGCCATGCGCATCTG GATGGACCCTGCGCTCATGGAGAAGTACGCGCTCATGCCGTCCGACCTCGTGAACGCGCTCAACGCGCAG AACGCGCAGGTCTCGGCCGGGCAGCTTGGGACATTGCCCGCGGTGCCGGGGCAGATGCTCAACGCCACGG TCACCGCGCGCTCCAAGCTCACGAGCGTGCAGGAGTTCAAGGACATCGTGCTCAAGTCCGCGCCCGACGG TTCGGTGGTCACCATGGCCGACGTGGCGCGCGTGGAGCTCGCGGCCGACTCGCTCACGGTGCGCTCCATC CTCAACGGCCAGCCCGGCGCGGGCCTCGGCATCGTGCTGGCCGATGGAGCCAACGCCATGCAGGTGGCCG ACGCGGTGGCCAGGAAGATCGCCGAGCTCGCACCCTACTTCCCTGACGGCATCACGGGCTTCGTGAGCTC GGACACCACGCCCTTCGTGCGCGCCTCGATCAAGGAGGTGATCAAGACGCTGGGCGAGGCCATGGTGCTC GTGACGCTGGTGATGTTCCTCTTCCTGCAGAACTTCCGCGCCACGCTGATCCCGGCGATCGCCGTGCCCG TGGTACTGCTGGGCACGCTGGGCGTGCTCTCGGCCATGGGCTACTCCATCAACATGCTGACCATGTTCGC GCTGGTGCTGGCCATCGGCCTGCTGGTGGACGACGCCATCGTGGTGGTCGAGAACGTCGAGCGCGTGATG AGCGAGCAGGGCCTGCCCCCGAAGGAGGCCACGCGCAAGTCCATGAAGGAGATCACGCCCGCGCTCGTGG GCATCGGCCTCACGCTGTCGGCCGTGTTCGTGCCCATGGCGTTCTTCGCGGGCTCGGTGGGCGTGATCTA CCGGCAGTTCTCGATCACCATCGTCGCTGCCATGGGGCTGTCGGTGTTCGTGGCGCTCACGCTCACGCCC GCGCTGTGCGCGTCGCTGCTCAAGCCCATGGCGCACGAGCGCACGGTGCGGCGCGGCCCGCTGGGCTGGG TCGACCGCTTCTTCATCTGGTTCAACCACCACTTCGACCGCAGCGCCACGCGCTACCAGGGCACGGTGGC GCGGCTGCTGCGCCGCGCGGGCCGCATGATGGTGCTGTTCGGCGCGGTGCTGGCGGCGGTGGCCTTCATG TTCCACCGCCTGCCCACCTCGTTCCTGCCCAACGAGGACCAGGGCTTCGTCGGGGCCACCGTCACGCTGC CCTCGGGCGCGACCGACGCGCGACTGATGGAGGTGATGGATCAGATACGCCTGTACTTCAAGGACAAGCC CGAGGTCTTCAGCACCAATGCCATCCTGGGCAACAACGGCAACCAGAGCTCGGGCAACATGTGGATACGC CTCACGCCCTGGGACAAGCGGCCGCTGCCCAGCCAGTCGGCCGAGGCCATCGCCAACCAGGCCACCAAGG ACCTGGCCAGCATCCGCGACGCGCGCGTCTTCGTCACGCTGCCGCCGGCCGTGCGCGGCCTGGGGTCCAG CGCGGGCATCAACTTCGTGCTCAAGGACCTGAACGGCCTGGGCCACGATGCGCTGATGAAGGCCATGAAC GACGTGATCGCCGCCTCGCGCAAGGAGCCAGCCATCATCAACATGCGCACCACGGGCTTCGACGACACGT CCGAGCTGAAGGTGGAGATCGACGACCGCAAGGCCGCCGCGCTCGGCGTGTCGGTGGCCGACATCAACGG CGTGCTCTCCAGCGCGCTGGGCGGCACCTACGTGAACGACTTCGTGCACTACGGCCGCGTCAAGCGCGTC TACATCCAGGGCGACGCGCCCTCGCGCATGCTGCCGCAGGACATCGCCCAATGGTCGGTGCGCAACCGCG ACGGGCAGATGGTGCGCTTCTCGGCCTTCGCCACCACCAGCTGGGGCAACGGCTCGCCGCAGCTCATGCG CTACAACGGCAGCCCGGCGCTGGAGATGCAGGCCAACACGCCGCCCGGCGCGAGCTCGGGCGACGCCATG GCCGCCGTGGAGCGCATCATGGCCACCATGCCGCCCGGCATCGGCTTCGAGTGGACGGGCGCCTCGCTGC AGGAGCGCCAGTCGGGCTCGCAGGCGCCCATGCTGTACGCCGTCTCCATCCTGTTCGTGTTCCTGTGCCT GGCCGCGCTGTACGAGAGCTGGAGCGTACCCTTCTCGGTCATGCTGGCCGTACCGCTGGGCGTGATCGGG GCGCTGGCCGCCACCTACGGGCGCGGCCTGGCCAACGACGTGTACTTCCAGGTCGGGCTGCTGACCACCG TGGGCCTGGCCTCGAAGAACGCCATCCTGATCGTGGAGTTCGCCGTGCAGCTGCAGGAGCGCGGGCGCAG CCTGATCGACGCCACCGTCGAGGCCGTGCGCCTGCGCCTGCGGCCCATCCTCATGACCTCGCTGGCCTTC GGCTTCGGCGTGCTGCCGCTGGCCATCGGCACCGGCGCCGGTGCGGGCGGGCGCCGGGCCATCGGCACCG CCGTGCTCGGCGGCATGGTGTTTTCCACGCTGCTGGGCATCTTCTTCGTGCCCGTGTTCTTCCTGATCGT GCGGCGCCTGTTCACCTCGCGCGGCGCGCCCGCGCACACCCCCCAGCCGGAGGCCGCGCCATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2455711-2458854	Sequence	Alide_2389 - transporter, hydrophobe/amphiphile efflux-1 (hae1) family
ORF... 2458851-2460359	Sequence	Alide_2390 - rnd efflux system, outer membrane lipoprotein, nodt family