Sequence retrieval

Genome: Alicycliphilus denitrificans BC
Start position: 2363715
End position: 2366948
Length: 3234

DNA sequence
>NC_014910, from 2363715 to 2366948 (3234 bp); Alicycliphilus denitrificans BC TCATTGCGCCGCTCCCTGGCCGCGCACCCGGTCCACGTGGTGGCGGTAGGCCATGAAATACAGGATGGGG ATGACCACCAGCGTGAGCACGGTGGAGACGGCGATGCCGAAGATCAGCGAGATCGCCAGGCCGTTGAAGA TGGGGTCGTCGAGGATGAAGAAGGCCCCCAGCATGGCCGCCAGCCCGGTGAGCATGATGGGCTGGGCGCG CGTGATGGCCGACTGCACGATGGCTTGCTTGAACTCCATGCCGGAGCGCACCTGCAGGCGTATGAAGTCC ACCAGCAGGATGGAGTTGCGCACGATGATGCCGGCCAGGGCGATCATGCCGATCATGCTGGTGGCGGTGT ACTGCGCGCCCAGCAGGGCGTGGCCGGGCATGACGCCGATGATGGTGAGCGGAATCGGCGCCATGATGAT CAGCGGCGTGAGGTAGGAGCCGAAGTGCGCCACCACCAGCAGGTAGATCAGGATCAGCCCCACGCCATAG GCCGCGCCCATGTCGCGGAAGGTCTCGTAGGTGATCTGCCATTCGCCGTCCCACTTGAGGCCGTAGCCGC GGTAGGGGTCGCTGGGCAGGCTGATGAAATACTCCTCGATCCTGCCGCCGTCGGGCGCGGTGATGTCCGC GATCGCCGCGCGCGCGGCGAACATGCCGTAGAGCGGGCTGTCCACCTTGCCCGCCATGTCGGCGACCACC AGGTTCACCGGCAGCAGGTCCTTGTGGTAGACGGGCTGCTCGCGCAGGGTGTCGGTGACGGTGACCAGCT CGCGGATCGGCACCGTGCCTCCCTGGCTGGTGCGTACGGGCAGCTGCAGCAGCTGGTCCAGGCTGCCCTG CTGCTCGGCGGGCAGCCGCAGCACCACGGGCGTGGGGTACTTGCTCGCATCGCGCAGCCAGGTCACGCTT TCTCCGGCCAGGCCCGCGTGCAGGGCGGAGACTATCGATCCCTGCGGCACGCCCATCAGCGCGGCCTTGC GCCGGTCCACCAGCACGAGCTTCCTTGGCGCATCGGCGATGGCGCTGTCGTCCAGATCCACGATGCCCCC GGTTTCTTGCAGCACTGCTCGCACTTCTTTGGCCACCTGGCGGCGGCCTGCGGCGTCGGGGCCGTAGATT TCGGCAACGATGGGGGAGAGCACGGGCGGGCCGGGCGGCACTTCCACCACCTTCACATTGGCGCCCAGCC TGCGGCCGATGTCCTGCAGGGCAGGGCGCACGCGCATGGCGATCGCGTGGCTCTGGTCCTTGCGCTCGTG CTTGTCCAGCAGGTTGACCTGCAGGTCGCCCACCTCGCCGCCGCTCCTCAGGTCGTACTGGCGCACCAGG CCGTTGAAGTTAATGGGGCCGGCCGTGCCCGCGTAGGCCTGGTAGTCGGTCACCTCGGGCACGGTGGCGA GATAGGCGCCCAGCTCGTGCAGCACGGCGGCGGTCCTCTCGGGCGGCGTGCCCGCGGGCATGTCCACGAT GACCTGGAACTCGCTCTTGTTGTCGAACGGCAGCATCTTGAGCTGCACCCAGCCCAGCACGGGCAGCACC AGCGATACGGCGATCAGCCCCGCCACTGCCAAGCCCAGCAGGCTGCGGTTGCGCCGGCCGTGCCGGTCGT CCAGCAGCGGGCGGAAGATGCGCGTGAACACCGGCGCGAGCCTGGCGGCCAATCCCGTGTGCGCCTGCGC CTGGCCGCCATGGCCTTTCATCCACAGCCGCGCCAGCCAGGGCGTGACGGTGAAGGCCACGGCCAGCGAC AGCAGCATGCCCATGCTGGCGTTGATGGGGATGGGCGCCATGTACGGCCCCATGAGCCCGGTGACGAAGG CCATGGGCAGCAGCGCCGCGATCACGGTAAAGGTAGCCAGGATGGTCGGCCCGCCGACCTCGTCCACCGC GCCGGGTATGAGCTGCAGCAGCGTCTTCTCGGGGTGCAGCGCCTGGTGACGGTGGATGTTCTCGACCACC ACGATGGCGTCGTCCACCAGGATGCCGATGGAGAAGATCAGCGCGAACAGCGACACGCGGTTGAGCGTGA AGCCCCAGGCCCAGGAGGCGAACAGCGTGGCCGTCAGCGTCAGGCCCACGGCCACGCCGACGATCAGCGC CTCGCGCCGGCCCAGGGCGACGAACACCAGCGCGACCACGGAGGCGGTGGCGAACAGCAACTTCTGGATG AGCTTTTGCGCCTTGTCGTTGGCCGTGGCGCCGTAGTCGCGCGTCTCGGCCACCTGCACGTCGGCGGGGA TGACCGTGCCGCGCAGCTCGGCCGCGCGCTGCATCACCGCGCGCGCCACGTCGATCGCGTTGGCGCCCGC CTTCTTGGTGACCGCCAGCGTCACCGCCGGGTATTCGCTGGCCTTGCCGTCCTCGACCTGCGCATGCCAG ACGTAGCGCGTCGCGGGCAGCGGGCCGTCCTCCACCCGGGCCACGTCCTTCAGGAACACCGGCTTGCCGC CGCGGTTGCCGACGACGATGTCGGCCACCTCCTGCGCGTGCGCGAGGAAGGGCCCGGCCTCGATGGACAC CGACCGGTCGCCCGAGAGCAGCTCGCCCACGGGCAGCCCCTGGTTGGCCGACTGCAGGGCCTGGCGCAGC GCGTCCACGGTCACGCCGCTGCCGGCCATGCGCGCCGCGTCCAGCTCCACGCGCACGGCGCGGCCGGGGC CGCCCACGGTCGCCACAGTGCGCGTGCCGGGCACGCGCATCAGCTCGGCCTCCATGCTGTGCGCCACGCG TTCCAGCGCAAAGGCGCCCGTGTCCTGGTCGTGGCTGTACAGCGTCAGCGTGACCACGGGCACGTCGTCG ATGCCCTTGGGGCGGATGATGGGCTCCATCGCGCCTAGGCCGCGCGGCAACCAGTCGGCGTTGCTGCGCA GCGTGTCGTGCAGGCGCACCAGCGCGTCGTTGCGGTGCACGCCCACCTTGAACTGCACCGTGAGCACCGC CACGCCGGGACGCGAGACCGAGGTCACGTGCTCCACGCCCAGCATCTGCGACAGCACCTGCTCGGCCGGC GTGGCGATCATCTGCTCCACGTCCGCCACCGACGCGCCGGGGAAGGGCACGATGACATTGGCCATGGTCA CGTCGATCTGCGGCTCCTCCTCACGCGGCGTGACCAGCACGGCGAACAGGCCCAGCAGCAGCGCCAGCAA CGCCAGCAGCGGCGTGATCTGCGCCGCCTGGAAGGCCTGGGCGATGCGGCCCGAGATGCCCAGGGAATGC TTGCGGTCGTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 2362297-2363718	Sequence	Alide_2302 - outer membrane efflux protein (Protein is coded in complementary strand; from 2363718 to 2362297)
ORF.. 2363715-2366948	Sequence	Alide_2303 - acriflavin resistance protein (Protein is coded in complementary strand; from 2366948 to 2363715)