Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 966301
End position: 967998
Length: 1698

DNA sequence
>NC_014814, from 966301 to 967998 (1698 bp); Mycobacterium sp. Spyr1 ATGCTGGACCGCCTGACCCGCATACAGCTGTCGATCTTCGCGATCGTGACCGTCCTGAGCGTGGGCGCGG TGGCGCTGTTCTACCTGCACGTGCCGGCCGCGGTCGGGATCGGCGCCTACAACGTGACGGCCAATTTCGT GGCCGGCGGCGGGATCTACCAGAACGCGAACGTGACCTTTCGCGGCGTCACCATCGGCCATGTCGAGTCG GTCGGCCTGTCCGACAACAGTGTGGTCGCACGCATGCAGCTCAACAGCGACACCCCGGTTCCGGAGAACG TGACGGCGACCGTCAAGAGCGTGTCGGCGATCGGCGAGCAGTACATCGACCTGGTTCCGCCGGAGGACGC GTCGCAGCAGATGCTGCGTGACGGCTCCGATATCGGCCTGGACCGCACGGCGATCGGGCAGGACATCGCC GGCCTGCTGGCCGAGGCCGACACCCTGGTGCAGAGCGTGAGCGACAGCAGGCTGCAGGAGTTGCTCGACG AGACCTTCAAGGCATTCAACGGATCCGGGCCCGAACTGGCCCGGCTGATCCAGTCGTCGCGGTTGCTCGT CGACGAGGCCAACGCGAACTACCCGCAGGTCGCCCAGCTCATCGACCAGGCCGGCCCCTTCCTGGACGCC CAGATCCGCAGCGGTGACGACATCCGTTCGCTCGCTGAGGGACTGGGGCGGCTGACCACGGAGGTCGCGG GCGCCGACCCGCAGCTGCGCACGACGCTGCAGACGGTGCCGGGCGCGGCCGAGGTGGCCAACGAGACGTT CGACGGAATCCGGCCGTCCTTCCCGATGCTGGCGGCCAACCTGGCCAACTTCGGCCGCATCGGCGTGATC TACAACAAGTCGCTGGAGCACGCTCTGGTCGTCTTCCCCGCGCTGCTCGCGGCGCTGAACACCGTCGCCG GCGGCGTGCCCACCGACGAGGGCGGCAAGCTGGACTTCAAGATCCACCTGCAGGACCCACCGCCGTGTGC GACCGGCTTCATCCCGGCGACGCAGATCCGCTCACCGGCCGACACCACCCTGCGCGAGCTGCCGACGGAC CTGTACTGCAAGGTGCCGCAGAACGATCCGAGCGTGGTGCGCGGCGCCCGTAACTACCCGTGCCAGGAGT TCCCGGGCAAACGTGCGCCCACCATCCAGCTCTGCCGCGATCCGCGCGGGTACGTGCCGCTCGGCAGCAA CCCGTGGCGCGGGCCGCCCGTCCCCGTCGGCACCCCGGTCCAGGACGGCCGGAACATCTTGCCGCCGAAC AAGTTCCCGATGATCCCGCCGCAGGTCGATCCCGATCCGGGTCCGCCGTCGGTGCAGTTGCCCCCCGGGG TCACACCGGGCCCCGGGCCCGCTCCGCACGCGCCGTTCCCGTTGCCCACGCCACCCAACGAGCAGGGCCC GCAGCCGGCGCCGTGGCCGTACTTCTCGCCGCCCGACCACGTGGTCCCGCCGTACGGCCGCCCACCGTTG GCGCCACCCCCGGCGCCCGGGCCGGCTCCGGCACCGGCTCCGGCACCGGGTGCCCCGCCGCTGCCCGCCG AGGCCCCCTTGGCCAGCTCCACCTCGGTGGCGACCTACGATCACAACGGCAAGTTCGTCGACCCGGCAGG CGGAGTCGGCGTGTACGCGCCCGGCTCCGACAAACTCGCGCCCGCGGAGAACTGGGTGGATCTGATGTTG GCGCCGAAGCAGGTATAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 965141-966301	Sequence	Mspyr1_09440 - virulence factor Mce family protein
ORF.. 966301-967998	Sequence	Mspyr1_09450 - virulence factor Mce family protein