Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 860649
End position: 863132
Length: 2484

DNA sequence
>NC_014814, from 860649 to 863132 (2484 bp); Mycobacterium sp. Spyr1 TCACGCCGGCGGCGGCGGGGGTGGTGCCGGTGGAGGGGGCGGCGGCGGAGGGGGCGCCGGCCACGGCAGC ACTATCGGCGGCAGCCCCGGGATCTCGATGACGTTCGGCGGAGGCGGTGGCGGCGGCGCGTCCCCGGGCG GTGGAGGTGGCGGTGCGCTGGGCGGTGGCGGTGGACGGTAGACCACGGGCGGGATGCCGTTGCTGGTGAG GATCGTGACAATCGAACCGGGGATCGTCTTACCGCGCGGTGACGTCCCCACCACCGCGCCCCTCGCGGCG CCGCTGTTGACCGCGGTCGGTTCGGGGGAGACCTGAAAGCCGCTCTCCGTCAGCTTGTTCCGAGCCGCGT CGAACTTCATCCCGGTGACGCTGGGCACTTCGTTGTTCGGCCCGCCGTCGACGTAGCGGGGCTCGGTGGG CGGCAGCGAGACCGGCCCGAAGTCCTCGGCGATCGGCTTCATGGCCCGGTACCAGGTCAGCGCGGGTTCG GTGCCGCCGTAGAGGTCGCCGCTGCCGCACTTGCGCAGCGGGTAGGAGCACAGGCCCGACGGGGACGGGG AGTCGTCGAAGATGTAGTTCGCGGCGGCGTAGCGATTGGTGAACCCGAGGAACGCCGAGGACCGGTGCGA CTCGGTGGTGCCGGTCTTGCCCGACATCGGAAGGTCCCATCCCACCGAACCCGCTGCGCCGGTGGCGGTT CCGCCGGTGTGGTCCTTGCCGAGCGCGTTGGCCATCGCGTTGGCGAGCCCTTCGGGCACCACCTGTTCAC AGGGCTGCGTCTGCAGCGGGACTTCGTTGCCGTAGCGGTCGAAGACTTTGTCGATCGGGCTGGGCGGGCA CCACACGCCACCCGAGGCGAGCGTCGCCGCGACATTGGTCAACTCCAGGGCGTTGAGCTCGAAGGGGCCC AGCGTGAAAGAGCCGATGTTCTGCCGCTTGACGAAATCGGCGATGCTCTCGTCGGTTTCGGGATCATAGA CACGGGCGGACCCGGGTTCGGCGTAGGAGCGCAGCCCGAGGCGGACGGCCATGTCCACCGCCCGGGGCAC CCCGATCTGCGAGATCAGTTTGGCGAACGCGGTATTGGGTGATTTGGCCAGCGCATCGGTGACGTTCATC TGGCCGGAGAATCCGGAGACGTTCTTCACGCACCAGGTCTTCGGAGGGCATCCCGGTTCGGTACTGTCGC CCAGGCCGGTGGCGCGAAAGGTCTGCGGCACGTCGAGGGTGGCGTTGATGCCCAGACCCATCTCCAGCGC TGCGGCGGTGGTGAAGATCTTGAAGATCGAGCCCGCACCGTCGCCGGCGAGGGTGAACGGTTGCGGCTGC ACCGTCTGCCCGGCGTTGAGATCCAGCCCGTAGCTGCGGTTGTCGGCCATCGCGACCACCCGGTGGGAAT CCTTGCCAGGGAGGATCACGCTCATCACGCTGGCCACACCGGGGGTGGTCGGCTCGGCGTAGTGGTCGAC GGCCGACTTCACGCCGGCCTGGATCTTCGGGTCCAGCGTGGTGCGGATCAGGTAGCCGTTGCGGGCGACG TCCTTCATGCTCAGGCCCGCCCGGGCCAGGTAGCGCATCGCGTACTCGCAGAAGAACCCCCGGTCGCCCG CGGCGATGCAGCCCTGGGGCAGCGGAGCCGGCCGTGGCAGCACACCCAGCGGTTGGGTCTTGGCGGCACG CAGTTCGTCGGCGTGCTCGGGCAGGTTCTCGATCATGGTGTCGAGCACGATGTTGCGCCGCGCCAGCGCG TCGTCGGGGTTGGTGTACGGGTCGAGGAGGCTGGTGGAGCGCACCATGCCGGCCAGCAGGCTCGCCTGTG CCAAGTCGAGATCGGCGGCGTCGATGCCGAAGTAGGTGCGTGCGGCGTCCTGCACCCCGAAGGCGCCGTT GCCGAACGCGACGAGGTTCAGGTAGCGGGTCAGGATCTCGGTCTTGGACAGCAGCTTGTCCATGGCCAGC GCCGCCCGGATCTCGCGCAGCTTGCGTGCGGGGGTGACGGCGACGGCGGCGCGCCGCTCGGCGTCGGTCT TCGCGCGCACCAGCAGGTTGAAGTTCTTGACGTACTGCTGTTCCAGCGTCGACCCGCCCCTGACACCTTT GTCGCCCCGCACGTAACCGGCCAGGCCGGTCAGGGTGCCCTGCCAGTCGACGCCGTTGTGTTCGGTGAAC CGCTTGTCCTCGATGGAGATGATGGCGAGCTTCATCGCGCCGGAGATCCGGTCACTGGGCACCTCCCAGC GGCGTTGCTCGTAGAGCCACGCGATGGGTTTCCCGGAGGCATCGACCATGGTCGACACGAGCGGCAGTTC GCCGTTGATCAGGTCCGCGGATTCGTCGGTGGCAGAGTTGGACACGCGCATGACCACAGTGCCCGCGCCG CCCACGAACGGGAACATCAGCGCGGCGACGAGCAGGCCCGCGACCAGGACGCAGCCGGCCAGTTTGCCGG CGGCGATCGCCTGTGCCCGACGTCGGTCGCGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 860649-863132	Sequence	Mspyr1_08610 - membrane carboxypeptidase (Protein is coded in complementary strand; from 863132 to 860649)