Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 80118
End position: 82112
Length: 1995

DNA sequence
>NC_014814, from 80117 to 82111 (1995 bp); Mycobacterium sp. Spyr1 TCAGGCAGCGACGAGGTCGGCGTCGGTGCGGTCGGCGGGCTTGATGAACTGCTTGGCCGCGACGACGGTC AGCCCGGCGACGACGGTGCCGACGACGAGCGCGATGACGAACCAGAGCAGGTTGCCGATCGCGAAGAACA CGAAGATGCCGCCGTGCGGGGCGCGCAGGGTCACCCCGAACGCCATGCACAGCGCGCCGGTGATGGCGCC GCCGAACATCATCGACGGGATGACGCGCAGCGGGTCGGCCGCGGCGAACGGGATCGCGCCCTCGGAGATG AACGAGGCGCCGAGAAGCCAGGCGGCACGACCATTCTCACGCTCGGGCTCGCTGAACAGTCCCGGGCGGA TGGTGGTGGCCAGCGCCATCGCCAGCGGCGGCACCATGCCGGCGGCCATCACGGCCGCCATGATCTGGAA GGAGCCGGTGGTGGCGGCGGCCAGGCCGGCCGTCGCGAAGGCGTAGGCGGCCTTGTTGACCGGGCCACCG AGGTCGAAGCACATCATCAGGCCGAGGATCACGCCGAGCAGCACCGCCGAGGTGCCGGTGAGGCTGTTCA GCCAGTTGGTCAGGCCGGTGTTGATCAGGGCGAGCGGGCGGCCGATCAGGAAGAACATGATCAGACCCAC TGCCAGCGACGCGATCAGCGGGATGATGACCACCGGCATCAGACCCCGCAACCATGCCGGCGTCTTGATA GTGCTGATCCACAGGGCCGCGAAGCCGGCGATCACACCGCCGACGATGCCGCCGATGAAGCCGCCGCCGA CGAACACCGCCAGTGCTCCCGCGGTGAAACCCGGTGCGAGGCCGGGACGGTCGGCGATCGCGAACGAGAT GTAGCCCGCCAGCGCAGGAACCAGGAAGCTGAACGCCAGCCCGCCCAGGGTGAACAGGATCGCGCCGAGG TACTGGAGGAAACCGCCGTCGGGGAGGTTGGTCAGCGAGTTGTTCAGCGCGATGTCGTTGCCGCTGTCGG CGATCTCGTAGCCGCCGAACAGGAAGCCCAGCGCGATCAGCAGTCCGCCGGCGGCGACGAACGGGATCAT GTAGCTCACGCCGGTCAGCAGGATCTGTCGGGTGCGGGTGCCCCACCCGACATTGCCCGACGGCGCGTCT GAGGCAGCCGCACCAGAGCCCTCCACCCGGGCGGCGTTCGGATTGTCCGCGGCGGCAACGGCTTCGGCGA TCATCTTGGCGGGCTCGTTGATCGCGCGCTTCACCCCGGAGGCGACGACGGGCTTACCGGCGAAGCGGCC TTTGTCCTTCACTCCGACGTCGGTGGCGAAGATGACCGCGTCGGCCTTGGCGATCGTGTCGGACGGCACC GGGGTGCTTCCCGAGGAGCCCTGGGTTTCGACGACCAGCGTCACCCCGGCCTCTTTGGCGGCCTGGCTCA GGGAGTCGGCGGCCATGTAGGTGTGGGCGATTCCGGTGGGGCATGCGGTGATCGCGACGATCGTCTTGGC CGCGGGCGGCGCTGCTGCGGGGGCGGCGGCGGGCGCAGCCGCAGCCGGGGCGGCCGGCGCCGGGTTGACG ACCCCTTCGACCAGGTCGACGACCTCGTCGGCCGTGGCGGCGTTGCGCAGCGACTCGACGAAGTCCTTGC GCACCAATGCCCGTGCCAGGCTGGACAACAGCTTCATGTGCTCCTGGCCGCCGGAGTCCGGCGCGGCGAT CAGGAACGCGAGATCGGCGGGGCCGTCCGGTGCGCCGAAGTCCACGGCGGGCTTCAGACGTGCGAAACCG ATGGTGGGGGTGTCGACATACGGTGAGCGGCAGTGTGGGATCGCGATGCCGCCCGGCAGACCGGTGGCGG ACTGCTCCTCGCGCGCCATCGCGGCGCCGACGAGTCCGGCGGCGTCGCCGGTGCGGCCGGCCGAGGCCAG GGCGTCGACGAGACGGGCGATCACCGCCTGCTTGTCCCCGTCGACCGAGGCGTCGAGCAGGACCATGTCG CGGGTGATGATGGGTCCTGGGGTGGAGCTGGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 80118-82112	Sequence	Mspyr1_00800 - PTS system D-fructose-specific transporter subunit IIABC (Protein is coded in complementary strand; from 82112 to 80118)