Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 699678
End position: 700340
Length: 663

DNA sequence
>NC_014814, from 699678 to 700340 (663 bp); Mycobacterium sp. Spyr1 ATGGCTGAGGCCAAGAACCAACTCGATGTCGACGATCTGAAGGCCCCGCTGCTCGCCGCCGTGGGTGCTG CCGACCTCGCTCTCGAGCGCGTCAACGAGATCGTCGCGACGCTGCGTGAGCGCGCCGGCGACGCCCGCAC CGACGCCGAGACCCGCGTCGAAGAGAGCCGCGCCCGGGTCGCGAAGCTGCAGGAAGACCTGCCCAACCAG TTCGGCGACCTGCGTGACCGGTTGACCTCCGACGAACTGCGCAAGTTCGCCGAGGGCTACGCCGAGGCCG CCCAGACCACCTACACCAAGCTGATCGAGCGCGGTGAGGCCGCTCTGGAGCGCCTGCGCAGCCAGCCCGC CCTCGAGGGCGCGGCCAGCCGGGTCGAGGAGTACACCGACCAGGCCGTCGAGCTGACGCAGGAGGCGCTG GGCAACGTCGCGTCGCAGACCCGCGCCGTCGGCGAGCGCGCCGCCAAGCTGGTCGGTGTCGAGCTGCCGA AGAAGGCCGAGTCGGCCGCCGAGCCGGTGAAGAAGGCCGCCGAGAAGGCTCCCGCCAAGAAGGCGCCGGC CAAGAAGGCCACCCCGGCCGCGGCTGCGAAGGCCAACCCGACCGTCCCGGCCAAGAAGGCCCCCGCCAAG AAGGCGCCGGCCAAGAAGGTCACCCAGAAGTAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 699137-699685	Sequence	Mspyr1_06820 - Helix-turn-helix protein
ORF.. 699678-700340	Sequence	Mspyr1_06830 - hypothetical protein