Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 620608
End position: 621309
Length: 702

DNA sequence
>NC_014814, from 620608 to 621309 (702 bp); Mycobacterium sp. Spyr1 TCAACCCCGGATGGATCCGGTGCGCGGAGGGTCGAGTGTTGCGATGCAGGTCACAGCCCGATCGCCGTTG TCCCAGGTCTCCGCGGTGGGGTAGAGGACGTAGAGCTGAACCGACTCGTCGAGCATCGACTGCGGCGAGT ACGCGACGAGCTCCGGACTGCATCTCTCGTGGTAGGCCTCGACCTCGGCCTGGCCCGGGAAGTCGCCGTC GGGCATCATCAGCACCGCGAAGACCTCGCCGGCGTGCGGTTCCTCGCAGCTGATCGTCTTGACGGTCAGA ACACGCTCCCCGTCGGGGATCTCGGCGAGGCAGTCACCGACCTTGACGTCGGTGGCGGTGACCCGGTCGC TGTTGGCGGCGAGCACCAGGAAGACGGCGATGCCGACGGTCCACACCGCCGCCAGCACGATGCCGGCGAT GGCCATGCCGCGGCCGCTCCGGTACTGCTTGGCCTTGATCAACCCGACGACACCGCACACGAGGGCGATC AGAACGCCGCCGATCACGCCGAAGATCAGCGAGACGATGGCCCACCAGTTGGTTCTCGGGGCCTCCCCGA AGGACGGATCCGAGGGATCGTTCGGGTAGGGCAGCGGCGGTGGCGGCGGGTAGGGCTGGCCGTACTGGGG TTCGCCGTACTGGGGCTGGCCGTAGGGTGGCGGCGGCCCCCACGGCGGCCCTGGCGGCGGAGGTACGGTC AC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 620608-621309	Sequence	Mspyr1_05950 - hypothetical protein (Protein is coded in complementary strand; from 621309 to 620608)