Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 5504958
End position: 5508119
Length: 3162

DNA sequence
>NC_014814, from 5504958 to 5508119 (3162 bp); Mycobacterium sp. Spyr1 TCAGAGCCGACGACGGTCGGCGACGGCATACGGCGGTGTCGCGGGCGTCACGACGTGATAGCCGAGATCC CGCAGGATGGCGAGTTCCATCTCGCTGAAGATCCGCACCCCTGGGCCCGTATCCGTCTTGGCGTTCATGA TCTTCTGGTCGTCACCGTCGAAGGTGAAATCGTCGAGGTGTGAGAACGAGCTGCCACCCTCGAACTCCCC CGGTGTGAACAACGGGATCAGATATCCGTCGTAGGCCTCGATCGCGTTCGATCCCCCGAAGTAGAGGGCC CCGTCATCCCCGGTCAGCTTGGGGTCGTTGAGGGTCGCCCACTCGGCGAGGAAGAACACCCTGGTACCGC GCACGTTGACCATCAATCGGTCGAACCTGCTGCGATTCGCGCCGAGATTCTCGCCCGGCTCACCCAACGA CGACAGGAACCCGAAGGAGTGCATGAGCTCATGGATCGCGGTCGTGACGAAGTCGTACTCATCGGCCGTC ACTTCACCGTCGAGCGCCCACTCATGGTCGGCGAAGTTCCACGAGATCGTGCCATCTGCTCTGTCGCCGT TGGCATCCCGACCGGTCTGCAGCTTGTGTTGCACGACGGTGTCCCAATAGCCGGGCAGCTTCGAGATGTA CGGACTCGTGGCCGAGGCGAGTCCCCGCTCCGGATCTTCCGGATCCTCGAGATTGACGTCGTAGGTCAAC GTGACGGGCCGGTTGACACGGAAGTACTCCTGCAGGTCCCGGGCTACCTGCTCCAGCGCGTCCTGACGAT CCTCGGTCCAGTCGTCACCGGTGTAGTTGAAGACGAACCTGATCGCGTTCTGGTTGATCAGGTTGAAGGC GCTGGTCCCGATACCGCGGAACGGGTTGAGCAGGTTCACGTGCAGACCGGAGTCGATGGCCATGACCCTG AACGAATCGACGCCGTCGAATGCCGCTCCCGGTGTGTAGGTCCACGTTCCGTCGACATTGAGGACGACCG AGCCCTGGCGGGGTCCGCTGACGATGCGGTAGCGGATGGCGTCACCGTCGGCATCCTCCCCGTTGATCTG TCCGGTGATCACGCCGGCGACAAGGCCGGTGACCTGAACCGGATCGACCGTCGGGGCCTGATTGAGGAAG GTCGTACGGAACGTCGCGAAGGACTCCTCCAGTTCCGCTTTGCGCTCGTCACTGACATCGAGGGAGTCGA TCCACTCCTGGTTGCGCGCGCTCAGACCGTCCACGATGTCGGCGACGACCTCGGCCCAGGTCCGGCGCGG GGACAAGGCGGTCGGTGCGGCCACCGGCGGCCGTGCCGGTTCTGCTTCGTCCTCGCCGCCGTCCTCTGCG CCGTCCTCTGCGCCGTTCTCCTCGGACTCCTCGTCCGTGGCGTACGGGGGATTCTCCGGGTTCACCACGT AATAGCCGAGATCCTCCAGGACGCCCAGCTCGACCTCGCTGAAAACCCTTGGCGCAGGACCTTCGTCGGT TCCCGAGTTCATGATCTTCTGGTCGTCGCCCGCGAACGTCGCATCGCTGAGATGGCTGATCGAACTCGCG CCGCCCCAGATCGTCGAGGTGCGCAACGGCACGAGGTACCCGCCGTAAGCCTCGACGGCGTTCTTGCCGC CGAAGTAGAGCCCGCGGTCACCGCCCGTGAGCTTCGGATCGTTCAACGACGACCACTGTCCGTTGGTGAA GACCGATCGGCCGTCTGCGCTCACGATGAACCGGTCGTACTCCTCACGATTCGCGCCCTGGTTCTCACCG GGCCCCTGCAGCGTGGAACCCCAGCCGAACGCGTGCATCATCTCGTGAATCGCCACCGAGGAGAAGTCGA ACTCCTCGCTGGAGACCTCGTCACCCAGCGCCCACTCGTTCCCGTCGCCCCAATTCCATGAGATCTCACC GTCGGCTTCTTCGCCGTTGGCGTCCTCACCGGTCTGCAGCTTGTTCTGCACGACCGTGCGCCAATAGCCC GCCGCAGTCGAAATACGTTCGCTGCCTGCCGATGCGAGCGTTCCGGGCTTGTCCTCCTCGTTCACGTCGA AGGTCAGTGTGACGGCCTCACGCACGCGGAAGTACGCAGTCAGGCTCGCTGCGACTTCCTCCAGTTTCTC CCGGCGTTCCTCGGTCCACTCGTCACCCTCATAGGTGAAATCGAAGCGGATCGCGTTCTGGTTGATGAGG TTTTCGCCCGTCGTGCCCATCCTCCGGAACGGGTTGAGCAGGTTGACGTGCAGGCCGGCGTCGATCGCCA TCACGCGGAAGGTGTCGACACCGAGAAAGCCCTCACCCGGGGTGTAGGTGTAGCCGCCGTCGTCGTCGAA GACGACAGTGCCGAATTTCGGCCGGGTCACCAATCGGTAGACGAGCTCGTCGCCGTCGGCGTCCTCGGCG TCGACCTTGCCGGAGATCGCGCCGCCCAGAAAGCCCTCGATCTGCACCGGATCGACGGTGGGCGCCTGGT TGAAGAAGGTTCGGCGCAGCGACCGCATCGCTGCTTCGAGATTGGCCTTCGCGTCGTCAGAGGCGTTGAG GTTGTTGATCCACGCGACGGTGTCGTCGTTCCATTTCTCAAGAACGTCGGCGACGACGTCGTCCCAGGTC TTGCGTTCCCGCAGCCACGGAGGTGCCGGCGCCGGTGGCTGTTTCACCTCGGCCGGCGGCTCCTCGTCCT CTTCCTGCACGGCCGCGTCGTCTTCCACAGTGACGGGGGCACTCGCCACCCGCACCAGATTCGGCTTCGC CGCGCGGTCCTGCACGCCCTCCGCGGCGGTGTCGATTTCGGCGGCGTCGCTTTCGGTTTCGGTGTCGGTG TCGGTGTCGGTGTCGGCCGTGTCGCGGCGGGAGTTGCTGCCCTTCGAGGAGGCCCTGTCGTCGGCATCGT CGTCGGCGTCCTCTCCGGTGTCGGTCGAATCGGGGGCTGCGTCGGTGGTCGACGAGGACTCGTCGTTATC CGCCGACGAGGACGCCCGCGGCGACGCGCTCGTCGTGTCCGTCGGACTGTCTGCTGCCGCTGTCCCGGTC GAGGGCCCCATCAGCGAGAAGCCCAGCAACGCCGCTCCCATTCCCGCCGACGCCGCGCCGAGCTTCAGCC ACTGCCTCACCGGGAAATCGGCGGACCTGCCCTGCGCTGAGTTCTGCTTGCGGCGTGCGTGATTCGTGCG CGACCTTGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 5504958-5508119	Sequence	Mspyr1_53110 - hypothetical protein (Protein is coded in complementary strand; from 5508119 to 5504958)