Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 5503216
End position: 5504874
Length: 1659

DNA sequence
>NC_014814, from 5503216 to 5504874 (1659 bp); Mycobacterium sp. Spyr1 CTAGTCGGCCTTCGCGTACACCAGATAGCCGATGTCGGTGAGAATGCCCAACTCGATGTCGCTGAGCGTT CGTACGCTGGGGCCACGGCTGGTCGCCGCGTTCATCAGCTTCTCGTCCCCATCGTTGAAGGTCCAATCAT CGAGGTGCGCGCCGGAACTCCCCTCGTCCCACGGGTCGGGCGTGAAGATGGGAACGTAACCGCCGTAAGC GTCCTCGGCGTGGGATCCACCCCAGTACAGACCGCCGTCCTCACCCTCGAGATACCCGTCGAACTCCTCC TGCCACTCGAAGTCGGAACTGATCGGGCTGGTCCCGTTCCGGGTGACGATGAAACTGTCCAGGATGTACC AGTGGCGACGGTCGCGGTCGCCGGGTTCCTCGGTCCCCGAGGTGAATCCGAACGCGTGCAGCAGCTCATG GATCGCCACGGCCCGGAAGTCGAACTTGTCGGACGGGACCTCGTCACCGATCTCCCAGCCCTTGCCCCAG TTCCAGTTGATCGAGCCGTCGGCCTCCTCACCGTTGGAATCCTCGCCACCGAGCAGCTTGTTCTGCACCA CCGTTCGCCAGTAGCCCGGGTCCCGGCTGATCGTGCCGCTGCCCGCCGAGGCCAACGTCGATGAGCTTGC GTTGTCGGACCCTTCGACCTCGAAGGTCAGAGTGACGGGCCTGGTCACTCGGAAGTACGCGACGAGATCG TCCGCGGCGTCCTGCAGGGCCTCCCGACGTGGCGTCGTCCAGTACTGCGAGCCGGTGGTGAAGTCGAAGT CGAAGGTGACGGCGCCCTGGTTGACCAGAGAGGTGGCTCTGCCGCTGCCCAGGGGCCGCAGCAGTTGCAG CAGGTTGACATGCAGGCCGAGATCGATGGCGCGCACATGGAACGCGTCGACGCCGTCGAACTCGGCGCCG GGTGTGTAGGTGTAGGTGCCGTCGGCGTTGATCTTCACCGTGCCCGTGGCCGGCGCCCTGGTGATCACGT ACCGCAGCCTGTCGCCGTCAGGATCGATGGCGCCGATCGTTCCGGTGACGGGTCCGGTGATGACGCCGGT GATCTGGACCGGAGCGATCGTCGGAGCCTGGTTGAAGAAGGTGGTTCGCATCGCCACGAACGACTCCTGC ATCCTCTCCTTGCGCTCATCGGTGACATCAAGGGAGTCCACCCACTCCTGGTTTCTCTGGGTCCATGCCG CGAGCGCTTCGGCGACCCGCTTCTCATAGGCGCTCTGCTCCGACACGTACGGAGGCTGGGTGGCTGCCCG GCCAGGGAGCGCGGTCGACGAAACCTGTTCTGCTACCGCAGCTTCCACGGCAGTGTCGGTCTTCGTGCGG TGCGTCGAGGGCTTGACCGCAACAGGCTCGGCGTCCGACTCCTCGGCGTCCGCGTCGTCCGCCGCCTCGT CATCGTCCGCCGCCTCGTCGGCGGCTTCCTCGTCGTCGGTTTCGTCGGCGTCGGCCGCCTCGTCGGCGTC GGTCGCAGCGTCCGGCGCGGTGGAGCCGCCATCCTCACCGGCGGAGGGTCCCGCATTGGTCGCGCCGGCG GACGCGGACTCGCTTCCGGGGTCCGCCGCCGCTGTGGCCGTCTCCGCGAGCACACCGAACCCGAGCAGGG CAGCGCCCATGCCCGCCGTCACGGCGCCGGCGTGCAACCACCGGCGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 5502323-5503219	Sequence	Mspyr1_53090 - hydrolase or acyltransferase of alpha/beta superfamily
ORF.. 5503216-5504874	Sequence	Mspyr1_53100 - hypothetical protein (Protein is coded in complementary strand; from 5504874 to 5503216)