Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 534913
End position: 536319
Length: 1407

DNA sequence
>NC_014814, from 534913 to 536319 (1407 bp); Mycobacterium sp. Spyr1 TCATGAACCGTCCTTGTCGAGGGCGAGTAGTGCGTTCTCCACGACCTCGGTCAGCGCAGGGTGGATCCAG TAGGGCCGCCGGGCCAGCTCCGCGGCGGTGATGCCGAAATTCAGGGCGACGACGAAGATCTGGACCAGTG TGGCGGCCTGCGGACCGATGACGTGCGCGCCGAGGATGCGCCCGTCCGGGGCGGCGAGCACTTTGCACAG ACCGGACTCGTCCTGCATCGCCCACCCGTAGGCGACGTCGGAGAACTCGGCGACCCCGGTCCGGTAGTCG AGATTCTCGTCGCGACAGTCCTTTTCGGTCCGCCCGATCGACGCGATCTGCGGGTGGGTGAACACCGCCG ACGGCACCAGTTCGTGGCTGACGGCACGCAGGTTGGCGGCGTGGAGCAGATTGTGTTTGACGACGGCGGC CTCGCGGTTGGCGACGTGTTTGAGCGGGATCTCGCTGCTGACGTCACCGAGCGCGAAGACCCCGTCGGAG GTGGTGCGGCAGTATTCGTCGACGACGACGCGGCCGTCCTCGTGGGTGTCGATGTCCCCTTTGGCCACGT CGAGGCGGTCGCTGTTGGAGCGGCGTCCCGCCGCGACCAGCAGCATGTCGGCGTCGACGATCGACCCGTC GTCCAGTCGGATACGCAGAGCGCCTGGCGCACCGGATATCTCGGTGACCTGACTCCCGCAACGCAGGTTG TAGCGGGAGCGCATCAGCTCGGTGTAGGTCGTGCGGACCGCGTCGTCCTGGGGGCCGCCGAGAAGTCGGT CGGAACGCTCGATCACCGTGATCCGGCTACCCGCCGCGGCGAAGACGTGGGCGAGTTCGGCCGCGATGTA GCCGCCGCCCAGCACGGCCAGATGGGCCGGCGGGGCGTCGACGCGCATCACCGTGTCGGAGGTCTCGTAG GGCAGGCCGGACCCGGCGACGACGTCGGGGATCACCGGCCGGCTTCCGGCGGCGATGACGATCCGGTCCG CGGCGATCTCGACGGTGTCCCCGTCGACGGCGACCTGCAGCCGGCCCGGCTGGGTGAACCGGGCGTGTCC GTTGTAGACGGTGATGAAATCGCTGTCCCGGCGGCCTTGTTCGCCGTCGGCGGCGATCGCGTCGGTGCGC CCGAAGACGCGGTCCCGCAATTGTGCCCACCGGAGCCGGCCGAAGTCGGCGTCGGTGTCGGTGTCGGCGT CGATGTCGATGTGGATGTCGAAGTCGCCCGCGGCCGCGACGGTGTCGGCGACCTGGGCGGTGTAGGACAG CATTTTCGACGGGATGCACCCGTAGTTGACGCAGGTGCCGCCGAACTTGCGGTCGTCGATGATGGCGACG TCGAGATCGGAGAAGCGGTCGTCGACGACCATGTTGCCCGACCCCGCGCCGATGACGACCAGGTCGTGTG TGCGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 533513-534916	Sequence	Mspyr1_05100 - glucose-6-phosphate 1-dehydrogenase (Protein is coded in complementary strand; from 534916 to 533513)
ORF.. 534913-536319	Sequence	Mspyr1_05110 - pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase component (Protein is coded in complementary strand; from 536319 to 534913)