Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 527526
End position: 529379
Length: 1854

DNA sequence
>NC_014814, from 527526 to 529379 (1854 bp); Mycobacterium sp. Spyr1 ATGCGCAGTTGTCTGGGTGTCTCGCTGGGCACAGCCAACCTCGTCGCCGTGGCCGACGGGCGGGCGACCG TCCGCCCCGCGGCCGTCACGGTGCCGTCGGGTCTGTCGGTGACGGGGTTCGTGGAACGGGTCGGGGATCC GGTGCCGTTGGTGCTCGCCGATCACTCGGTCCACCGGCCGGAGACGCTCGCGGCAGCCGCGGTCGAGGAC CTGACCCGCTGGGCCAGCCCGCAGCGGCGCCCGGATCTGGTCACGGTCGCGGTCCCCGCCCACTGGTCCG GCGCGGCGATCGGTGCGTTCCGGTCCGCGGCTCCGCACGTCACGGTCGTCTCGGAGGTGTCGGCCGCGGT GACGGCACTGCAGGCCTCACCCGGACTGCCGACCCGCGGCGTGATCGCGCTGTGCGATCTCGGGGCGGGG GGAAGCAGCATCACGCTGGTGGACGCCGGCAACGGGTTCGCCCCTGTCGGGGACACCGTGCGGCTCGACG TGCTCTCCGGCGACGGTGTCGACGGCGCGGTCTTGCAACACGTCCTCGCCGAGATCGAGACCGACCCCGG CGGCACCGCGCACGTCGCGGCATTGTCGGGGTTGCGCGACCGGTGCCGGGCCGCCAAGGAGCGGCTCTCT GTCGAGACGGCGACCGGACTCGTCTGGGGCCGCACGACGGTCCGACTGACGCGCCCCGAGCTCGAAGCCC TGATCGACGACGCCGTCGGCGGCTTCGTCGACGCGGTCGTGACCACGCTCCACCGGTACGGCGTCTCGCC CGCCGCGCTCGCCGCGGTGGCGACCGTCGGCGGTGGGGCGCGGATACCGCTTGTGACCGAACGACTTTCG GAGGCTCTGCAACGCCCGGTGATCACCACCCCGGGTGCCGCACTCGCGGCGGCCGCTGGTGCGGAACTGC TCGCGCGACGTGGCGCGGAGCCGGTGGCGCAGACGGTGTCGGTGGCGGCGCCGATGACGGCCGCGAACGC GACGGTGGCCGCACCGGTGACGCCGCTGGCGTGGTCGGCAGAGGCAGACGATCCTGGGGCGCACGCCGTG TTCGAGGACGAGACGGTGTACGCCACCGCGTACGCGCGCCCCGATGTCCATTTCGAACCCGACGAGATCG ACGAGGCCGACGTCGAACCGCTCGGGTGGTACCGCCGGCCCGGCGTATTGTTCGCCGCTGCAGCGTGTCT GGCCGCGGTCGCGACGACCGGGCTGGTGCTGACCGCTCAGGTCGGACCCGTGGAAGCGGACGCCTCGGAC ATCGCGGCGCCGGTGGCGACCGCGCCGGTGCAGGCTCCGACCATGCCGGTGGAGATCGCACCGCCGCCCC CGCCCGTCACCGAGACCGTCGCGGCAGCCCCGGCGCCACGGGTCGTCCAGGCCGCCCCGCAGTCCCCGGC CCCGCAGCGGGTGGTCGCGAAACAGGCTGCGCCGCAGGCGGTGCCGCGTCCGGCAGCACCGGCTACGGAG ACGGTCGCGGCTCCCGCGCCGAGCACCACGCCGACCCCGACACCCACTCCCAGCTCGACGCCCACGCCGA CGCCCAGCTCGACGCCCACGCCCACGCCGACGCCCAGCTCGACGCCCACGCCCACGCCCACGCCCACGCC CACGCCGACCCCGACACCCACGCCGACCCCGACACCCACGCCGACCCCGACACCCACCTCGACGCCCGCG CCTACCTCGACGCCGCCGCCGGTGACGTCACAGCCTGTGCCCGTCGAGACACCGACCGCGACTCAGGAGC CGGCTCCTGCCGAGACGCCGGTGACGGAGATCCCGGTCACCGAGACGCCGGCCACCGAGACGCCGGCCAC GGAGTGTGTGCCGACGGCGGAACTCGCCTGCTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 527526-529379	Sequence	Mspyr1_05040 - hypothetical protein
ORF... 529380-529901	Sequence	Mspyr1_05050 - hypothetical protein (Protein is coded in complementary strand; from 529901 to 529380)