DNA sequence >NC_014814, from 5220633 to 5220707 (75 bp); Mycobacterium sp. Spyr1 CCCGGGGCTGACCACTCCCGGCCTGACCACTCCCGGTCTGACCACGCCCGGCCTGACCACGCCCGGTCTG ACCAC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI