Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 5220116
End position: 5221090
Length: 975

DNA sequence
>NC_014814, from 5220116 to 5221090 (975 bp); Mycobacterium sp. Spyr1 GTGCCGAACCGACGTCGCCGCCGGCTCTCGACAGCCCTGAGCACAGTCGCCGCTCTGGCCGTCGCGAGTC CGATTGCAGTTATCGCCGTTTCCGAATTGTCCGCGTCGACCCTGGCAGGCCGCGACGGAGCGCCCCAGCA CCGCGAATTCGTCCAGGCTGCCACCATCACCGATCTTCCGGGTGAGCTGATGACCGCCCTGTCGCAGGGG CTGTCCCAGTTCGGGATCAACCTGCCGCCGTTGCCGACGGGGCTGCTCACCGGAACCGGGGCATCCTCGC CGACCACCCTGTCACCGGGACTGGGTACCGCCGGCCTGACCTCACCCGGCCTGATGCCGGGACTGTCGGC CGCACCGTCGCTCACCGACCCCAGCCTGGCCAACCCGGCCCTGACCAGCCCGATCGGCGCCACGCCCGGT CTGACCACGCCCGGCCTGACCACACCCGGTCTGACCACGCCCGGCCTCACCGATCCGGCGCTGGCCTCAC CCGGCCTGACGACGCCGGCACTGACCGACCCGGGGCTGACCACTCCCGGCCTGACCACTCCCGGTCTGAC CACGCCCGGCCTGACCACGCCCGGTCTGACCACGCCGGCCGCGCTGACGGATCCGGCGCTGGGCCTGCCT GCCACCGGCGAGATCCCGATCTCGGCGCCGGTCGGCCTCGACCCGGCGCTCGGCTCGTACCCGATCCTGG GCGACCCGTCGCTCGCCGCGACCCAGCCGGTCGCGACCAGCAGCGGCGGCCTGCTCGGCGACCTGACCAG CGCGGCGAACTCGCTGGGCGCCGGCGAGGCCATCGACCTGCTCAAAGGCGTCCTGATGCCGGCGATCATG TCCGCGGTCAAGCCGCCGGTTCCGCCGGTGCCGGCCGCACCGCTGGCCACCGCCGCGGGCGAGGTGGCAC CCGTGGAGGCCGGACTCGGCGCAGCCGAGGCCTCCTTCGAGACGGGCGCGGAACCGGGCGCCTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 5220116-5221090	Sequence	Mspyr1_50290 - GLTT repeat protein