Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 5146500
End position: 5147678
Length: 1179

DNA sequence
>NC_014814, from 5146500 to 5147678 (1179 bp); Mycobacterium sp. Spyr1 GTGGGTCGGCACAGTCTGTCGTACGTCACTGAACTGCAGGAATGTTCCAGCGCACCGGTGCGGCGGTCCA CCGCCGGCCACGTCGGCCGGGTGGGACTACTGGCGGTCGCGTTGGGAATCGGTGCGGCAGCGGTGGGCGG CACCGCGGTTGCTGGTGCTGACACAGAGTCCAGCAATTCCGCCCGATCGTCGGCGGTATCGGCCAAGCCC AGCACGGACCGGACGGCCGACGACTCCGCCGACACATCACAGCGCGGTGGCTCCCGGGCAGACGACGACC CCGCCTCGGCGCCGACGACGGCGCTCGCCGGTGTACGACGCGACGCCGAGGACACCCCCGACGCCGACGA CCCTGACGCCGGAGACGTCGCGGCGGCGAAGGCGGCCGTGGAAGCCGACGCGGACGACGATGCTGCGGAC GGCGACGCTGCGGAGGGCGATGCTGCGGCCCCGGACCCCGATGATCAGGTCGACACCGAGTACGGCGACA TCGGCAAGTGGATGCTCGATTCCCGTGGTGAGATCGCCGACTACGGCGGACTGCCGTACGAGGGCCGCAC GGTGCTCGAACCGGTCAACGTCGTGATCGTCGACTCGCGCTCACGCAGCACACTGGAAGCCACCTGGCGA CTCAACGCGGCCATGTGGCGGGCCGGTTTCCCACCGCGGCTGATCCACAGCACCGGGTTCCGCGGGCTCA TCGACAGTCAGCTCTACCGTCAGCAGCCGCGGGGACTCCTGGTCGGCTACTCCGACGCGTTCTTCCTCCT GCCGAACAACCACGGGCGGATCTTCGGGCCCGACCCTGTCGAGACGAGCAGCGGCTACGTGTGGAGCGGT GCGTTCAGCACCGAGGAGTTCGTGTTCTACCAGGGCCTGCCCCGGCACGCGTATGTGTCGGGGAATGACG CCAGGGACGCGCTGGCGGCGAAGCTCGTCACCAGCGGTCGGGCCACACTGGGAACGCCTGTCTCGCTGGA GAATTCGTACAACACCGACACAGTGACCACCGGGGACCACGACGGCTACGCGGCGGTCCTGATGCTGAAC AACCTCACGACGTTCGCAGCTTCGGCTTCGGCCTCGGACTCGCGTCGCTGCGCGCCGGCGAGCTACTTCG CCGTCGCGGGATCGTCGTGCGCGGCACCGGGAGTTCCTGCCGAGTCCGGATCGCGGTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 5146500-5147678	Sequence	Mspyr1_49650 - hypothetical protein