Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4963306
End position: 4966131
Length: 2826

DNA sequence
>NC_014814, from 4963306 to 4966131 (2826 bp); Mycobacterium sp. Spyr1 TCAGGCCTTCTTGGCCGCTTTCTTCGCCGGCGCCTTCTTGGCGGCCTTCTTCACTGCGGTCTTCTTCACT GCGGCCTTCTTCGCGGCCTTCTTCTTCACCGGACCGCGCGCACGGCGGTCGGCGAGCAGTTCGGATGCGC GCGCATCGGTGATCGACATGACGTCGTCGCCCTTGCGCAGACTCGCGTTGGTCTCGCCGTCGGTGACGTA CGGGCCGAAGCGGCCGTCCTTGATCACCATCGGCTTCTCCGAGACCGGGTCCACGCCGAGTTCACGCAGC GGCGGGGTCGCCGCACCCTGGCGGCCCCGGCGCTTGGGCTCGGAGTAGATCTTGAGGGCCTCCTCGAGCG TGATGTCGAACATCTGCTCTTCGGTCGCCAGCGACCGGGAATCAGTGCCGCGCTTCAGATATGGGCCGTA GCGGCCGTTCTGCGCGGTGATCTCCTCACCGTTGGCGGGGTCGACACCGACCACGCGGGGCAGCGAGAGC AGCCGCAGCGCGTCGTCGAGGGTGACGGTCTCCAGGTCCATCGACCGCAGCAGCGAACCGGTACGCGGCT TGGGGCCGGTCGGCTTCTTGCCCTTCTTGGCCGTCGCCCCGGCCTCACCGTCATCGGGCGGCTCGGGAAG GACCTCGGTGACGTACGGTCCGTAACGGCCGTCCTTGGCGACGATCTCGTGTCCCGTCGCCGGGTCGATC CCCAGCGAACGCCCCTCTTGCGGTGTGGCGAACAGCTTCTCGGCCAGCTCCAGGGTCAGCTCGTCGGGCG TCAGCTCGTCCTTGAGGTTGGCGCGCTGCGGCTTCAGCTCACCCGGATTATCAGGGTCCGCAACCATCCG CTCCAGATACGCGCCGTTGCGGCCGACGCGGACGTTGACCGCGCGGCCTTCCGAATCGTCGAACAGCTTG ATGGAGTTGACTTCTCGGGCGTCGATCTCTTCGAGGTTGCCGCCGACCAGTTTCTTGAGCCCGCCCTCCC GGGCGATCGAGCCCTCGACTCCGTGCTCGCCGCCGAAGTAGAAGTTGTTGAGCCAGTTGGTCCGTCGCTC GTTGCCGGCGGCGATCTCGTCGAGCTCGTCTTCCATCGCGGCGGTGAAGTCGTAATCGACCAACCGGCTG AAATGCTGCTCCAGCAGGCCGATCACGGCGAACGCCACCCACGACGGGACCAGCGCGCTGCCCTTCTTGT GGACATAGCCGCGGTCCTGGATGGTCTTGATGATCGAGCTGTACGTCGACGGCCGGCCGATTCCGAGATC TTCGAGAGCCTTGATCAACGACGCCTCGGTGTAGCGCGCGGGCGGCGAAGTGGTGTGGCCGTCGGCGGTC AGGTCCTTGGCGTCGACACGCTGACCCTGGGTCAGGTTCGGCAGCCGGCTCTCGGCGTCGTCGGCCTCGC CGCCGGCCTGCTCGTCGAGACTCTCGACGTACGCCTTCAGGAAGCCCGCGAAGGTGATCGTGCGACCGCT GGCGTTGAAAACGACCTGCTCGCCCGTGCGGGCTTGGCCGGCGATGCGCAGGCTCAGCGTGGTGCCGCGC GCGTCGGCCATCTGGGAGGCGACGGTGCGCTGCCAGATCAGCTCGTAGAGACGGAACTCGTCGGTGTCGA GCTGGCTGTGCAACTGGCCGGGGGTCTGGAACACGTCACCGGCGGGGCGGATGGCCTCGTGGGCCTCCTG CGCGTTCTTGACCTTGCGGGTGTACTGGCGCGGCGTCGGGTGGACGTACTCCTCGCCGTAGAGCTGGCGG GCCTGGTTGCGTGCGGCGTCAATGGCCGACTGCGACAGCGTCGTCGAGTCGGTACGCATATAGGTGATGT AGCCGTTCTCGTACAGACGCTGCGCGATGCTCATCGTGCGCTCCGAGGACATCCGCAGCTTGCGGCCGGC TTCCTGCTGCAGCGTCGAGGTCATGAACGGCGCGTACGGGCGGCGGGTGTAGGGCTTCTGCTCGACCGAG GAGACCGACAGCTGGGCGCCGCGCAGACCACCGGCCAGCGCGTTCGCGGCGGCTTCGTCGAGGACGAGCA CCTCACCGGGCTTCTTGACCGCGCCGAGGGAGTCGAAATCGCGGCCTGCGGCCACGCGGCGGCCGTCGAC GGTGTTGAGCTTCGCGACGAACGTGGGCGGTGTGGCCTGCGCGTCGGACACGCTGGCGTCGAGTTCGGCG GTGACGTCCCAGTAGCCGGCGCTGCGGAACGCCATCCGCTCGCGTTCGCGCTGGACGATGATGCGCGTCG CCACCGACTGCACGCGGCCGGCCGACAGCTTCGGCGCGACCTTCTTCCACAACACGGGGCTGACCTCGTA GCCGTAGAGACGGTCCAGGATGCGACGGGTCTCCTGGGCGTCGACCAGGTCGTTGTCGAGGTCGCGGGGG TCCTCGGCGGCCGCACGGATCGCGGGCTCGGTGATCTCGTGGAACACCATCCGCTTGACCGGGATGCGCG GTTTCAGCGTCTCCAGCAGATGCCAGGCGATCGCCTCGCCCTCGCGGTCACCGTCGGTGGCCAGATAGAG CTCGTCGACGTTCTTGAGCTTGTCCTTCAGATCGGCGACGGTGGCCTTTTTATCTGGGCTGATGATGTAG AGCGGCTCGAAGTCGGCGTCGACGTTGACGCCGAGGCGCGCCCACGGTTCCGACTTGTACTTGGCCGGGA CGTCGGCGGCCGCGCGCGGCAGGTCGCGGATGTGCCCGCGGGAGGACTCGACGACGTAGTTCGACCCCAG ATAACCTGCGATTTTGCGCGCTTTGGTCGGCGACTCGACTATGACGAGTCGCCGCACGGACCCGTTCTTG CCGCTGCCGCGGTCCCCGTCAGCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4963306-4966131	Sequence	Mspyr1_47780 - DNA topoisomerase I (Protein is coded in complementary strand; from 4966131 to 4963306)