Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4932522
End position: 4933037
Length: 516

DNA sequence
>NC_014814, from 4932522 to 4933037 (516 bp); Mycobacterium sp. Spyr1 TCAGCCGGCGCTCCGGGGCCGCAGCACCAGTTCGGTGAGGCGCACACCGGCGCGCTCGGCGGGATCGAGC GCGGCCAGGTGCGCCGACACGGCTGAGGCGGCCGAGGCGGCCGAGGCGTCGACCGCCGACCACGGCACCA GCACGAAGGCCCGCACGTGGGCATAGGGGTGGGGCAGGGTCAGGTCGGGATCGTCCATCACGATCTCGGC GTCGCCGTCCCGGCAGGAGACCAGATCGACGTCCAGTGTGCGCGGCCCCCAGTGCTGTTCGCGGGTCCGT CCGCACGCATCCTCGAGCTCGTGGGCCCGGCGTAGCCACCCGTGGGCGTCGAGTGCCGGATCCTCGGCGA GGACGACCGCGTTGAGGAACGGGCTCTGCTCGACGCCGCCCCACGCATCGGTCTCGTAGACCGGCGACAC CCCCGTCACCGCCGGCCCCAGCGCGTCGAGCACCGACTGCAGATGCGCGAGTCGGTCACCCAGGTTCGAG CCGATGGACAACACAACCGAGGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4932046-4932522	Sequence	Mspyr1_47500 - hypothetical protein (Protein is coded in complementary strand; from 4932522 to 4932046)
ORF.. 4932522-4933037	Sequence	Mspyr1_47510 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (Protein is coded in complementary strand; from 4933037 to 4932522)
ORF... 4933034-4933405	Sequence	Mspyr1_47520 - dihydroneopterin aldolase (Protein is coded in complementary strand; from 4933405 to 4933034)