Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 491897
End position: 492370
Length: 474

DNA sequence
>NC_014814, from 491897 to 492370 (474 bp); Mycobacterium sp. Spyr1 CTAGCGGTCCTTCGGCGCGAACGCTGCCGTGAAGGAGTCGCGGCGTGGGAATGTTTGTGCCGCAGCACCG GATTGGAACCCTGCCGGTTTGTGGCGGGTGATTCCCCAGTGCGCGTGCGCCGCCAGAAGACTCTTGGTCA GCTCGTCGACCCGTTTGGGGTCGATGTTGCCGTCGCCGTCGACGAGGCTGTCGATGGTGGCTCCGTCGCG CCAGACGTCGGCGGGGTCGGCGAGGCGGTCTGCGACGAGGCGTTCGACTTCGCCGCGCTGCAGGATCTCG AGGCGGGCTTTGAGGGTGTCTCGTTCGGCCTCAGCGGTTTGTGCTCGGCGGCGGTACTTTCGGGCTTCCT TAACCGCGTCGGGGGTGCCCTCGGTGGAGTCCTGGTCGGGCTTGTCGACCGTCGTGTCGGTGTCCTCCGG GGTGGTGGATTCTTCTGGCGTGGCCTCGGTGGCCTCGGTGGCGTCGGTCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 491897-492370	Sequence	Mspyr1_04710 - hypothetical protein (Protein is coded in complementary strand; from 492370 to 491897)
ORF... 492367-492630	Sequence	Mspyr1_04720 - hypothetical protein (Protein is coded in complementary strand; from 492630 to 492367)