Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4777133
End position: 4778353
Length: 1221

DNA sequence
>NC_014814, from 4777133 to 4778353 (1221 bp); Mycobacterium sp. Spyr1 CTAGGGCGCAACGTAATACGTCGTATTCCCGACGACCGCACGCACCATGAGGGCCGCGACGTCGACGTTG TCGTACCCCCGGTGGTCGGTGAACATGCCGAGGAAGAGCCTGATGAGTTCGGTGCCGCTCTCGCGGGTTA TGTTGGGCCAGTTCTCCCAGTTCGCCCAGCCGTCCCAGCGCTTGGCGATGTCACGGACGGTGTACGCCGT GTCCTCCGGGGTCAGCGCGACCCTGCGGAAGTTCCAGCCCATCAGGTCCGGGTTGGAGCCGAGCCGGCGA GAAGGGTTGCCCAGCAGCGTGAACCGCATCCGGTCCGGCTCGGGAGCATCCGCGTCCTGCGCGAAGCTCG ACAGCCACTCCCCGACGACCTGGGCACCCTGCGACATCCCGATCACGTCGACGAATCCCGTGGTCGACCG CAGCAGCCGGTCGAGGTTGGCGGCGCCCTGCGTGATCGAGCTCTGCCGCGCGGCCGCCGGGTAGACCAGC GGAATCCAGGTGTTCCCCGGGGATTTCGGGTAGCCCTGCAGATAGTCGCCCGCCTCCTCCGGCGTGGATC CCAACCCTCCGATCGTGACGACGGTCCGCGTCTCGGACGGGACGTCGGGGGCTGTCGGACTGTGCACCGG AAGCGGGCTGCTGTCCGGCTCTTCCAGAGGGGCGGTCGCCGGTATGTCGGGATCGTCGACAGTGGGCTTG CGGGGGTCGGTCACCTGGCTTCCGGCCGTCGCATCCGTGCCGGATCGGCCGGGTTGGCGCCACGCTGCCG TCAGGACCGTCCACAGGGAGGTCGACCGGGCAGGTGGTTCGGGACTGTTCGCGGCGGACGTCACCGGGAC ATCGGCATCGGCATCGGCATCGGGCGTCCGGTCCTCCGCTGCGTCGTCGGAGATGTCGCCGTTGGAGATG TCGCCGTCGGAGATGTCGTCGAGCCTGTCGGACTTGCGGGCGCGCGCCTCGGAGACGCGCGACGACGACG GCTTAGCCTGCGCTCTGGTGCGCGCCGCCGGCGCCGTGGCAGGTTCGTCGCGATCCGGGCTGTTCGTGGC CGGTCCCCGGGACGGGCCGCCGGCGGCGTCCGCCGACGCGGCGGCCCGTCGCGAGTTGCTGACGGCGGAG TCGTCGGCATTCGCCGTCCCCGCCGCACTCCCCAGCCCGATACCGATGCCCAGCGCAAGTGCCAGCGCGC CGATCCGTCCGACATGTCGTGCAGAACTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4776147-4777136	Sequence	Mspyr1_45980 - hypothetical protein
ORF.. 4777133-4778353	Sequence	Mspyr1_45990 - PE-PPE domain-containing protein (Protein is coded in complementary strand; from 4778353 to 4777133)