Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4543668
End position: 4544765
Length: 1098

DNA sequence
>NC_014814, from 4543668 to 4544765 (1098 bp); Mycobacterium sp. Spyr1 TTGGCTGCGCTGGATTCATCGGTCACGTCGCGCACACAGGCGCGTGCCGACCACTTCCGTTCGGGCCTTC TGTTCGCACTCGCCTCCGCCTTCGCATTCGGCTGCTCCGGCCCCTTCGCGAAGGCTCTGATGACGGCGGG CTGGAGCCCGACTGCGGCGGTCACCGCACGCCTGGCCGGCGGAGCGCTCGCGATGGCCGTCTTCGCGACC GTCGTGCGCCCGGGATGGGTGCGCGAGGCACGCCACCACGCGAGGACCGTCGTGCTGTACGGGATCGTGC CGATCGCCGGCGCCCAGCTCTTCTACTTCAACGCGGTCGCGCACCTGTCGGTCGGTGTCGCGCTGCTGCT CGAGTACACCGCGCCGCTGCTGGTCGTCGGCTGGTTGTGGGCCACCACCCGGCATCGTCCGAGCGGCATG ACCCTCGCCGGCGTCGTGCTGGCCGTCGGCGGGATCACGTTGGTGCTGGGGATCATCGGGCCCGGCGGAG TGGCCGCGGCGCACATCAACCTCGCCGGCGTCGGCTGGGGGCTGGCCGCCGCCGTGTGTGCGGCCTGCTA CTTCCTGATGTCGGACAAGGCGGGCGCCATGTCGGACAAGGAGGTCGCCGACGGTCGTCCCCCGCTGCAC CCGATCACGCTCGCGGCCGCGGGCCTGGCCTTCGGCGCCGTGACGGTGGCCGCGCTCGGGTTCTCCGGCC TGATGCCCCTGGCCTTCACGGCCAACGACGCCGTGATCGCGGGCTTCACGACACCGTGGATCGTGCCCGT CATCGCACTGGCGCTGATCCCCACCGCGATCGCGTACACGCTCGGCATCGTCGGGATAGCCCGCCTGCGC CCCCGGTTCGCGTCGATGGTGGGACTGTCCGAGGTGATGTTCGCGGTGCTCGCCGCCTGGATGCTGCTGG GTGAGGGGATGGCGGCCGCACAGGTGATCGGCGGGGCGATCGTGCTGGCCGGGCTCGCGCTGGCCCGCCA GGGCGACCGTGGTGAGCTCGTCGGGGAAACCAACGTCGGGGAAACGTCGGTGACCCCGACGGCACTGCCG CAAACCACCCCGGTCGCGCGCGGTGGTCCCGCTACCATCGACCCGTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4543668-4544765	Sequence	Mspyr1_43810 - drug/metabolite transporter permease
ORF... 4544762-4545439	Sequence	Mspyr1_43820 - Cutinase