Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4522842
End position: 4524251
Length: 1410

DNA sequence
>NC_014814, from 4522842 to 4524251 (1410 bp); Mycobacterium sp. Spyr1 CTACCCCGCCGACTCGCCGACCTGCGGCACTTCACCGGCGGCGATCTCACAGGGAGTCTGTTCATCCGAC GCCGGCTCGGCCGCAGGCACCGGCTCGGCGGGCGGGGGCGGCGCAGGCGGCTCGGGTGCCGCGACCGTCG CCGCCGGCTCGGGGGGCGGGTCGCAGGGCTCCTCAGGCAAGACCGGGTCCGCAGCCACAACGGATTCCGG CTTTTCCTCTGCTTCGTCGTCCTCTTCAAGCTCCGCGTCTTCTTCGTTCTCCTCCTCCTCAAGCTCCGGC TCCTCAACCCCGGGCTCGTCCAACGCCGGCTCTTCCAGGTCCAGGTCCGGGGATTCGAGCGCGTCTCCCC CGAGGCCACCACCGAGCAGACCGCTCAACGCGTCGGCGAAGTGCTGACCGAAACCCGACGGTCCGCCACC GAGACCGGGCATGCCCGATCCCAGGGAGCCGGGCGACGGCAGGGGCGCCTCGGGTGCAGGCGCGGCGAGA GGTGCAGGCGCGAGAGGTGAAGGCGCGGCGGGCGCAGGGGGGACGGGCGCCGGCGCGGCGGGCGGGCTCC ACGCGGAGGGTGCCGTGGCGGCCGGCGTCCCGGCCGGCATCCCCGCCGGCGGACTCCATGCCGCAGACGT GGCTGCCGCCGGTGGGCTCGCCGCGACGGCTGCCCGGCCGGACTGCCCCTCGTCGTCGCAATTCGGTCGC GGCTCAGGACAGTCGGGTCCGAGGTCACCCGGAACGTCGAACGTCGGGGTGCGTTCCGCCGCGATCTCGG CGGTCGCCCCGTCGTACGCCGCGGTGACAGCAGCCACGGCGTCCCGCATCGCGGGGAGCCACTCGTCACG AATCACAGTGTCCACGAAGGGTTTCACCGCGTGCTCGACGATCTCGCCAGCTGTCGCCGTGTCACCGGTC CCCGTCGTCACGGACGTGGCCGCCGCCGACCACTGCGCCTGCGTACCCGCGGCGCGACCTTCGGCGGAGA CCGTCGAGTCGACTTTCCCACCGACGACCCGCCACAACTCCTCGCGCAGCGATACCAGCGCCTCCGCGGC GGTACGGACCGCTGCCGTCGCCACGGCCGACGAATCACCGTGTCGGGCAAGGAACATCCGGGCCGCGTCA CCCCCGGCGCCCTCCCACGCCGCCGACAACTCCCCGAGTTGACGCTCCTGCACGGCCAGTGCCTCCTGTG CTGCGCGCCCGACCCCGTCGAGCGCCACACAGCAGCGGTGCAACGCTGCGAGGTCCATGCCGTCCTCGGT GCCGTACCAGTCGGTGACCTGCGCGGGGTGCAGGGTCAGGTCGCGATGCTCGTAGCCCAGTTGCCGGCAG GCCGCGACGTAGCGCTGCAGGGTGTCGGCCGCGGGACGTCCCTGCGCCAACCGGGCGCCGACGTCGAAGT CATCCGGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4522842-4524251	Sequence	Mspyr1_43620 - hypothetical protein (Protein is coded in complementary strand; from 4524251 to 4522842)