Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4440622
End position: 4442319
Length: 1698

DNA sequence
>NC_014814, from 4440622 to 4442319 (1698 bp); Mycobacterium sp. Spyr1 ATGGCGACCAACACCGATCTCTTTCGCAGCGCCCGCGACCACCTGGTCGACGTGATGGCCGACTACGACA AGGCGCTCGACACCTTCGAGTGGCCTCGGATCGCCGGGTCGTTCAACTGGGCCACCGACTGGTTCGACGC GATCGCCGCAGACCCGGCGTCCGCCGACCGTCCGGCGCTGTGGATCGTCGAGGAGGACGGCCGTGAGCAG AAGGTCACCTTCGCCCAGATGGCGCACCGCTCCGACCAGGTCGCGACCTGGCTGCAGGGTCTCGGCGTCG GCAAGGGTGACCGCGTCATCCTGATGCTCGGCAATCAGGTGGAGCTGTGGGAGTCGATGCTCGCCGTCGC CAAGCTCGGCGCGGTGATCATGCCGACGACCGGAGCCCTCGGTTCCGAAGACCTCGCCGACCGGATCCGG CGCGGCGGCGCGGGCTACGTCATCGCGAATGCGGCCGACGCGCCGAAGTTCGACGGCGTCGACGGCGACT ACGTCCGCATCGCCGTCGGGGGCGGCGCAGACGGCTGGCACACCTACGCCGACTCCGCCCACGCCGACGC CCACACCCACGACCCGCAGACCACCGCCGGCGACCCGCTGCTGGTCTACTTCACCTCCGGCACCACCAGC AAGCCCAAACTCGTCGAGCACTCCCAGATCAGCTATCCGGTCGGGCACATGACGACCATGGCGTGGCTCG GCGTGCGGCCCGGCGACGTCCATCTGGCGATCAGCGCGCCCGGCTGGGCCAAACACGCGTGGAGCTGCTT CTTCGCGCCGTGGATCGCGGAAGCGACGATCTTCGTCTACAACTACGGACGCTTCCAGGCACCGGCCCTG CTGGACCAGATCCGGCGCGCCGGGGTGAACACGTTCTGCGCGCCGCCGACGGTGTGGCGCATGCTGATCC AGTCCGACCTCGGCGAGCGCCCCGAAGGTCTGCGCGAACTGCTCGGCGCCGGCGAGCCGCTCAATCCCGA GGTGATCAGCGCGGTCGAACAGGCGTGGGGGCTGACCATCCGCGACGGGTTCGGCCAGACCGAGACGACG CTGCAGATCGGCAACACTCCCGGCCAGCCGGTCAAGGCCGGCTCGATGGGCCGCCCGATGCCCGGTGTGC CCGTCGTGCTGGTGGATCCGCTGACCGGAGAGCTCGCCGACGAAGGCGAAATCTGCCTCGACCTGAGCGG GACGCCGCTGAACCTGATGACCGGGTACCTCGGCGACGACGAGCGCAACGCCCAGGTGATGCGCGACGGT TACTACCACACGGGCGACGTCGCAAGCCGGGACGCCGACGGCTACATCACCTACATCGGACGCACCGACG ACGTCTTCAAATCCTCGGACTACAAGGTGTCGCCGTTCGAGCTGGAAAGCGTACTCATCGAGCATCCCGC GGTCGTCGAGGCCGCCGTGGTCCCCCAGCCCGACGACACCCGCCTGGCCGTGCCGAAGGCCTACATCGCG CTGGCCGACGGATGGTCGGCCGACGAGAGCACCGCCAAGGCGATCCTCGAGCACTCGCGCGACCATCTCG CCCCGTACCTGAAGGTGCGGCGGCTGGAGTTCGCCGACCTGCCCAAGACCATCTCGGGCAAGATCCGCCG TGTCGAACTGCGTCGCCGCGAGGAGGACGCGCACCGCTCCGGTGCGCCGATCGAATCCGAGTACCGCTAC GAGGATCTGGTGAACTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4440622-4442319	Sequence	Mspyr1_42820 - acyl-CoA synthetase/AMP-acid ligase
ORF... 4442319-4443935	Sequence	Mspyr1_42830 - acyl-CoA synthetase