Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4335937
End position: 4337454
Length: 1518

DNA sequence
>NC_014814, from 4335937 to 4337454 (1518 bp); Mycobacterium sp. Spyr1 TTATTCGGGAGCTTCGCGCTTGCGGCGCTTGCCAGGGCGCGCTGCGCCTTCCGGTGGCTGCATCGACGCA GATACCCCACCGTCGCTCGTTATTTTAGTCGAGCGACGCGCAAGTACTTCTACCGGCGGAGGAGTAGTAC GCGATGGCCGCTTGCCGACGTCCTCACCATAGTAGCCGTAGCTGTAGCTGTAGCTGTAGGAGGAGCCTCC GCGGGACGGCGTCATCGTGAACACGGCACCAAGCAGCGGTGCGCCAACGCTTTCCAGGCTTGCGACGGCG TGCGTTAGGTGCTCTCGCTTTGTCTGACCGAATCGCGCCATTATCAGCACGCCGTCCGCACCCGCTGCCA GAATCGCGGCATCTGTGACCGCAAGCAACGGCGTCGAATCTACGATCACATAGTCAAATTGCCCTCGCAG TTCATTTAGCAGTTTTCTGGCCGCTTGCGATCCAAGAAGTTCGCTCGGATTTGGCGGAATAGCGCCAGCC GCCAGCACCGTCAACCCAGGGAAGCGCGTCTTTTGGAGTGCCTCCGCGATCGGTGCTGCGCCACTAAGTA CAGTGCTGAAGCCGACGGTACCGACGAGATCTAAGTATTTATGTAGCATTGCCCGCCGCATATCGCCGTC TACTAGCACGACACTGTGCTCTGCCTCGGCTAAAGCGAGTGCTATGTTGATTGCAGTTGTCGATTTACCC TCGTGAGGCATGGAGCTTGTTACGACGATTACTCGCGGAGGATTGTCGACGGCTAAGAATTGTAAGTTAG TACGGAGCTTACGAAATGCTTCGGCGATCGGGGAATTATCTTGTTCGTAAGAAATGGCCGGCTGGTTGCG TCGAGCCCTATCAAGGGGGATGCTTCCTACCATCCCAGTCCCAGTGATTTCTTCGAGTTTTTCTTTAGTC TTAACGGTGTTGTCTACAACATCTCGTAGAACCGCGAGGCCAATTCCGAGCACCAAACCAAGCACCAAAC CAGTGACGACGTTGCGGAACGTCTTAGGGACGACGGGTTGCTGAGGAATTGATGCACGCTGTTCTACGAC AACCCGGGAGTCCGGATCGCTTCCGTCGGCGGGCGCTTCTAGTTCCCGTACCAGAGATACAAACTCTTCG GATAACGCATTGGCTACATCACGAGCCCGAACCGGTGATTCGTCGAGAACTAATACGTCGATCAGAACGG TATTAGGTTTCGCGCTAGCTGAGACATTCGCTCGCAAGGCGCTTGCGCTCATATTAAGGCCGAGTTTGTC GATAGTGCGCTGCGCCAAGGTCTCGCCTGTAAGCAGTTCGGCGTACGACACAACCCTATCTTGGGAAAAT CTATTTCCTTGGTATGTGTCGGCTAGCGACGATCCAGCAGCGGTAGACACGAAGAGCCGCGTTGATGCCT GATATTGCGGAGTAGTCAACAATGAGACAGCCACAGCCCCAAGCACTGTGGCGGCAAGCGTCACGCCCAC GGTAACCCACCGGGAGCGCAATAGTTTGACGAAATCTTGGAAATTCAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4335937-4337454	Sequence	Mspyr1_41800 - capsular exopolysaccharide biosynthesis protein (Protein is coded in complementary strand; from 4337454 to 4335937)