Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 4066486
End position: 4067757
Length: 1272

DNA sequence
>NC_014814, from 4066486 to 4067757 (1272 bp); Mycobacterium sp. Spyr1 TCAGGAGTCGGACGTTGCGCTGGGCTTCGACGGGCTCCTGTCGGTCGATGCGCCGGAGTCGGAGCTCACC GAGCCGTCCTTGTCCGCGTCGTCACCACTCTGGGCACCGGCATCCGAATCCTCCTGCGCCGCTTCTTTTT CCGCACCCGAGTCGATGTCGGCCTGGTCCGCGGTGTCGGCGTCCTCGATGTCCGCGTCCGCGGAGTCGGC ACCCTTCACGTCGACCTCCTCGGACACCGCGGCCTCCTCGGATACCACGGCGTCCTCGACCGCGGCGTCC TCGGATACCACGGCGTCCTCGACCGCGGCGTCGGCCTTCGCCGTCCGCAGCGATGCCACGACGCTACCCG GCACCTGGGCCGCAGAGGGGGTCTGCACGACCGCCGTCGGGGCGGCTGGCGCCGGCGCAGCAGCGAAGAC CGCCGCCTTCGGCGCAGACACGGCCACGACGTCGTTGCGGCTGTAGGCGGCGTCGATCTTGGCCTTCAGT TCCGCTTCACGGTTGGCCAGCGACGGGAACAACCGGACCAGCGGAAGCTTCTCGGCCGGCACCAGGTAGT GGGTGGTGGTCCCGCCCTCGGAGTTCACCTCGACCGAGATGTTCCTGGCCGGAACCTTCGACAGGTCGGC GTACATCACCGGGATGTGAACGAAAATGCCACCAGCGACGGCGTTGACGATCGCCAGCAGGTTGAGGCGA TCCGGGAAGTCGGCCATGCCGTCGTACTCACCGGTCACGACGATGATGTCGTACTTCGTCTCGGGAGCGG TCTGGTAGGTGTAGTCGAGGTTCGCGTTGTACCGCGTGCGGCTGATCACCTTCTGGCGGCTGGAGTCGGC GACCACCACGAAGGTGATCTTGTCCTTGCCGGGGGCGTCTGCGCTCTCGTCGAGTTCGCGCAGAATCTCG TTGACGACGAGGGACCCCGCCGACAGACCGACGACGGTCACCTTCTCGCCGCTCTTGAGCTGCGCGAGCG CGGCATCGATCTGTGCGTTGAGGTTGGTCGCGCCCTGCGCGATCGACGCGCCGAGGGTGAGATCGCCCTT TCCGGTCATCGGACCCGCCTGCGCCGGCCACTTCACACTGACGCGCTGCTGATCCTTGAACTTGCCGCCC AGCAGCGGAGACATCAGAGCATCCGCCATCGACGGCGTCGAGATGCCACCGATGACGAGCGCTGTGTTGG CGGCCTGGCCCAACGAGGACGACGACAACGCCAGTGCCGCCGAGGCAAAGACGGTGCCGCAGACGACACC TGTTTTTCGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 4066486-4067757	Sequence	Mspyr1_39180 - PE-PPE domain-containing protein (Protein is coded in complementary strand; from 4067757 to 4066486)