Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3991109
End position: 3991645
Length: 537

DNA sequence
>NC_014814, from 3991109 to 3991645 (537 bp); Mycobacterium sp. Spyr1 CTAGGGCTGTGGCGGCGTGGGCGGCAGTACCGGCCCCGGCGGGGCGACCGTCGTCGGGGTCAGCACCGGC GGCGCCTGGGTGAACGTCTGCGGCGCGAGCGGTCCCGGTCCGTCGGGGTCGATGACCGTCGTCGTTGGCT CCGACGGGAACAGCGGTCCGGCAGGCGGTGGCGTGGATGTCTCCGGCGACGTGGTCGGCGTGTCCGGTGT CGGCGACGGGGTCGTAGTGGTGGTCGTGGTCGGGGTGGTCGTGGTGGTGGTCGTGGTCGTCGTGGGCTCG GGCGCGCGGGTCTCGACGTTGGTGCGCGGCACCCACGTGTAGTTCGGGTCCGGCACGAAACCGGGCGGCA CCACCTGCTGAGCCGGATCCATCGCCGGCGCCGGCGGCTCGGGTTGATAGTTCTGGTTCACCCAGTACAA CGCGAAGAAGACCAGGACGAGCCCGACGGTCGATACCCGCATGCGACCGCCGAAGAGATAGGCCCGTGAG TGACGGTTCTTCGGCTGCAGCCGCTGCAAGAAGGATGGAAGGGTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3991109-3991645	Sequence	Mspyr1_38480 - hypothetical protein (Protein is coded in complementary strand; from 3991645 to 3991109)
ORF... 3991642-3992613	Sequence	Mspyr1_38490 - small-conductance mechanosensitive channel (Protein is coded in complementary strand; from 3992613 to 3991642)