Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3866772
End position: 3867419
Length: 648

DNA sequence
>NC_014814, from 3866772 to 3867419 (648 bp); Mycobacterium sp. Spyr1 TCACGCGCTACGGCCGACTGACGACTCCCTGACCTCGGCCCGGTCGCGCACGCCGAGCAGCATGCGGCGC TCCATGATGAAGTGCACGACGTCGTAGAGCACGTTTCCGCCGTATCCGCCGTTGCCGCGTGCCAGCAGAC GCGTGCGCCCGCCCTGCGCCTTGAGATGGAAAGACCAACTCCCGGAGGCTTTCTCGCCGCGCTGCACCCG TTCGTAGTCGGCCGGGGACATCAGGACGAGGTGTGAATTCGGCATGAGAGCGGCGACGATCTGCCTGCCC CGGTCGCCGCCGCGGCGTGCGAGCCAGACGGTGTCGCCCACGGCGAGATCCTGCCATTCCCGGTGGATCC TCGACGCATTGCGGATGTCCGCCCCGACAGCCCGTTCGAGCACGCTGTAGCTGTAGAAGCCTCCGCGGTC CTCACCGATCTGCGCGAGCCACGGCCACACCTGCGTCGGCGGGGCATCGATGGTGATCGCCCGCGTGGTG CGAGCCCCTCCGGCGACGAGTTCGTCACCGGGCAGACCGGCCACCGACTCCTCGTAGGTGGCGCCCCACC GGTATATCCACGGCCGCAACCGGGTGCGATACCCGATCACCGTCGCTGCCAGAGCGAGAGCGGCAACGCC CGCACCGGAAGCTTTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3865661-3866803	Sequence	Mspyr1_37290 - acyl-CoA dehydrogenase
ORF.. 3866772-3867419	Sequence	Mspyr1_37300 - hypothetical protein (Protein is coded in complementary strand; from 3867419 to 3866772)
ORF... 3867416-3868303	Sequence	Mspyr1_37310 - universal stress protein UspA-like protein (Protein is coded in complementary strand; from 3868303 to 3867416)