Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3719184
End position: 3720812
Length: 1629

DNA sequence
>NC_014814, from 3719184 to 3720812 (1629 bp); Mycobacterium sp. Spyr1 ATGACTTTCTCGAGCCCCTTCCCCGAAGTCGACATCCCCACGGCCAGCGTCTACGACTACCTCTTCTCCG GCCTGTCCGATCCGGACGACCCCGTCCTCGACCGCGTCGCGCTGATCGACGCGAAGTCGGGCAGGCAGAC CACCTACCGCGAGATGGTCGCCCGGATCGACTCGTTCGCCGGGGCGCTGGCCGCCCGGGGCGTCGGAGTC GGCGACGTCGTCGGCCTGCTGGCGCCCAACAGCTCGGCCTTCGCGGTGGCGTTCCACGGCATCCTGCGCG CAGGGGCGACGGCGACGACGATCAACGCGCTGTTCACCGCCAAGGACATCGCCAAGCAGCTGACCGATTC CCGGGCCCGCACTCTCATCACCGTCACACCGCTGCTGGCCCAGGCCCGGGAAGGTGCGGCGCTGGCGGGG CTGTCGGACGAGGACGTGATCGTGCTCGACGGACCCGGCCGCGACGGGTCCGGCGACGGCGCGGGACACC CGAACGCCGCCGACATCCTCGCACCGGGAACGCCTGCGCCACAGGTGAACTTCGCACCGTCGTCGCATCT GGCGGTGCTGCCCTACAGCTCGGGCACCACCGGGAACCCGAAGGGCGTGATGCTCACGCACCGCAATCTC GTCGCCAACGTCGCGCAGATCCGCCCACTGCACGGCATGGAGCCCGACGATGTCGTGCTGGCCGTGCTGC CGTTCTTCCACATCTACGGAATGACCGTGCTGCTCAACGCCGCCCTGCATGCGCGTGCCCGGCTGGTCAT CATGCCGAGCTTCGACCTCGGCGACTTCCTGGGCAACATCGCCGAACACCGGTGCACCATCGCGTTCATC GCTCCTCCGGTCGCGGTCGCGCTCGCCAAGCACCCGCTCGTCGACGACCATGATCTGTCCTCGTTGCAGG TCGTCATGTCGGGTGCGGCGCCACTGGACGCCGATCTCGGCCACGCCGTCGCAAAGCGCCTGGACTGCAA GGTCGTTCAGGGATACGGCATGAGCGAGCTCAGCCCCGTCAGTCATATCACCCCGTTCGACGGCGGCCGG CTGAACATGCACGACGACGCTCCGCTCGCGTCGGTCGGGTGGACGGTGTCCAACGCGGCCTCCAAGCTCG TCGACCCGGAGACCGGCGCCGAGATCGAGATCCCCGACGAGGGCTTGAGCAAGACCGGCGAATTGTGGTT CAAGGGCCCGAATGTGATGGCCGGATACCTCAACAACGAGGAGGCCACCAAGGAGACCATCGACGAGGAC GGCTGGCTGCACACCGGCGACCTCGCCCAAATCGACGACCGGGGCCTGGTGTACATCGTCGACCGGCTCA AGGAACTGATCAAGTACAAGGGCTACCAGGTGCCTCCCGCCGAACTGGAGGCCGTGCTGCTGTCCCATCC CGACATCGCCGACGCCGCGGTGGTCGGTGTGCGCGACGAGGAAGGCGAGGAGGTCCCCAAGGCCTTCGTC GTCACGCAGGCGAACTCCGAGCTGACCGAAACCGACGTGATCGAGTTCGTCGCGGGCCAGGTCGCCCCCT ACAAGAAGGTGCGCAAGGTCGAGTTCATCGACGCCATCCCGAAGTCGGCGTCAGGCAAGATCCTGCGCAA GGATCTGCGGACCGGTTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3719184-3720812	Sequence	Mspyr1_35960 - acyl-CoA synthetase
ORF... 3720809-3722620	Sequence	Mspyr1_35970 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein (Protein is coded in complementary strand; from 3722620 to 3720809)