Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3691101
End position: 3691754
Length: 654

DNA sequence
>NC_014814, from 3691101 to 3691754 (654 bp); Mycobacterium sp. Spyr1 CTACTTGCGTCCGCGCTTGGCCGGAGCCTTCTTGGCGGGCGCCTTCTTGGCCGGAGCCTTCTTGGCGGGC GCTGCCTTGGTCGCGGCCTTCTTGGCCGGAGCCTTCTTGGCAGGTGCTGCCTTCTTGGCGGCCGGAGCCT TCTTGGCCGGTGCCGCCTTCTTGGCGGCGGTCTTCTTGGCCGGTGCCGCCTTCTTGGCGGGTGCGGCCTT CTTCGCCGGAGCCTTGGTCGCAGCCTTCTTGGCAGGTGCTGCCTTCTTGGCTGCGGTCTTCTTGGCGGGC GCGGCCTTCTTGGCCGGAGCCTTCTTGGCCGCCTTGCGGGCGGTGCTCGCAGCTGCGACGCCGCGCTTGA CCGCCGGGCCTTCGGCCGGAAGCTTCTGCGCGCCAGACACAACGGCCTTGAACTGAGCACCGGGACGGAA CGCCGGGACGGAGGTGGGCTTGACCTTCACCGTCTCGCCAGTGCGCGGATTACGCGCCACTCGGGCTGCG CGACGACGCTGCTCGAAAACACCGAAGCCGGTGATGGTGACACTCTCACCTTTGTGCACCGCGCGGACGA TGGTGTCGACGACATTCTCCACCGCCGCAGTCGCTTGCCGACGATCCGAGCCCAACTTCTCCGTGAGTAC GTCGATGAGCTCTGCTTTGTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3691101-3691754	Sequence	Mspyr1_35680 - nucleoid protein Hbs (Protein is coded in complementary strand; from 3691754 to 3691101)