DNA sequence >NC_014814, from 3675679 to 3675753 (75 bp); Mycobacterium sp. Spyr1 GGCGCGGCGATCGGCGGTGGTGCGATCGGCGGTGGTGCGATCGGCGGCGGGGCGATCGGAGGCGGGGCGA TCGGC Translate to protein Restriction digest BLAST Design primers with primer3 Genome related info at NCBI