Sequence retrieval

Genome: Mycobacterium sp. Spyr1
Start position: 3458646
End position: 3461315
Length: 2670

DNA sequence
>NC_014814, from 3458646 to 3461315 (2670 bp); Mycobacterium sp. Spyr1 CTAGAAATCCGTTTCGTCGTCCTCGCCGTCGGCGCCGTTGGCGTCACTGCCGCCGCGGATCAGGGCCAGC GTCCCGGCGAGCTCGTCGGGCTTGACCAGCACCTCACGCGCCTTCGATCCCTCCGACGGGCCCACGATGC CGCGAGTCTCCATCAGGTCCATCAGCCGGCCCGCCTTGGCGAACCCGACGCGCAGCTTGCGCTGCAGCAT CGACGTCGAACCGAACTGCGACGACACCACCAGCTCCACGGCCTGCAGCAGCACGTCCATGTCGTCGCCG ATGTCGGGGTCGACGTCCTTGCGTTCCCCGGCCTTGACCGCGGTGACGCCCTCGACGAACTCCGGCTCGG CCTGGTCCTTGGTCGCCGTGACGACCGCGTGGATCTCCTCGTCGGTGATGAACGCGCCCTGCAGCCGGAT GGGTTTGTTGGCGCCCATCGGCAGGAACAGGCCGTCGCCCATGCCGATCAGCTTCTCGGCGCCGGGCTGG TCGAGGATGACGCGGCTGTCGGTCAGCGACGACGTCGCGAATGCCAGCCGCGACGGCACGTTGGTCTTGA TCAGACCCGTGACGACGTCGACCGACGGTCTCTGCGTGGCCAGGACCAGATGGATGCCCGCCGCGCGTGC CTTCTGCGTGATCCGCACGATCGCCTCTTCGACGTCACGCGGCGCGGTCATCATCAGGTCGGCGAGCTCG TCGACGATCGCCAGGATGTACGGGTACGGCTTGTAGATCCGGTTGCTGCCCAGCGGCGCCGTGATCTCAC CGGACCGCACCTTGTCGTTGAACACGTCGATGTGGCGCACCCGCGAGGCCTGCATGTCCTGGTAGCGCTG CTCCATCTCCTCGACGAGCCAGGCCAGCGCCGCGGCGGCCTTCTTCGGTTCGGTGATGATCGGCGTGATC AGGTGCGGGATGCCCTCGTACGGGGTGAGTTCCACCATCTTCGGGTCGATCAGGATCATCCTGACCTCGT CGGGGGTGGCCCGGGCCAGCAGCGACACCAGCATGGAGTTGACGAAGCTGGACTTGCCCGAGCCCGTCGA GCCGGCCACCAGCAGGTGGGGCATCTTCGCGAGGTTCGCCGAGATGAAATCACCCTCGATGTCCTTGCCC AGACCGATCACCAGCGGATGGTGGTCGCGGCGGGTCGACGGCGCGGTCAGCACATCGGCCAGGCGCACCA TCTCGCGATCGATGTTGGGCACCTCGATACCCACCGCCGACTTGCCCGGGATCGGCGCCAGCATGCGCAC GCTCTCGGTGGCCACGGCGTAGGCGATGTTGCGCTGCAGCGCGGTGATCTTCTCGACCTTGACACCCGGC CCGAGCTCGACCTCGTAGCGGGTCACGGTCGGCCCCCGGGTGCAGCCGGTGACGGCGGCGTCGACCTTGA ACTGCTGCAGCACCGAGCTGATGGCGTCGGTCATCTGCTGGTTCGCCGCGGTGAGGCGCTTCGGCGGCTC CCCGGCCACGAGCAGATCGAGCGGGGGCAGCACGTAGGGTCCCTCGACCACCCGGTCCAGCGAGAGCTTC GGCTCTTTGGGCGTCTTCTTGCGTGACGTGACGGCCGGCGCGGCGACGGGCGCGGCGACGTCGTCGTCCT CGTCGACCAGCGGGTAGTTCTCCATCGGCGTGCCGGTCGGGCCGTCGGGACGGGTCAGCGGCACCGTGGG CTGCGCGGCGGCGGGCCACTGAGCCTCGTCGTCGGGGTCGCCGATCTGCTCGTCGTAGTACCCGTCGGAG AAGTCGTCGGCGCGGTCGTCGCGGTCGCGGTCGTCGTCGCCGTCGTGGTCCTCGTCGTAGTAGTCGTCGC CGTCGTAGGACTCGTCGCCGTCGTAGGAATCGTCGTAGTACTCATCGTCGTCCTCGCGCCAACGCGTGGT GAACATGTCGCGCACGGTCGCGGGGACCTCGCGGATGGTCGTCCCGGTCAGCAGCAGCACCCCGAACAGC ACGCCGATGAACAGCAGCGGGGCCGCGATCCACGCGGTCAACCCGTCCGACAGCGGTCCGCCGAGCGCGA AACCGAGGAATCCGCCGGCGTGCCGGCGCTCCACCGGGTCCGCCGGCGCCCCTGCCCAGAGGTGCCACAG TCCGAGTGCGGGCAGCGCGACCATCGCCGAACCCAGGATCAGCCGCGGCCTGGCCTCGGGGTCCGGCTCC GAACGCATCAGCGTGACGCCGACGACGGCGAGCAGCACCGGCACCAGCACCACCGCGGCGCCGATCAGCA CCCGCACGGTCGTGTCGATCCACGCGCCGACCGGCCGGGCCGCGTCGAACCAGGAACTCGCCGCCACGAT GACCGCCAGTCCCAGCAGGGCCAACGCGATGCCGTCACGTCGGTGGCCGGGTTCGAGTTCGCGGGCCCGT CCCACCGAGCGCGCGGTGCTTCCCGCCCCCTTGGCCACCATCAGCCAGCCGGCGCGCACACCTTTGCCGA CGGCGGTGCCCGCCGTCGAGATGGCCGAAGAGTTGCGTCGAGCAGGCTTGCGCGGAGCCGGTTTGCGGCG GGGCGCGGCAGGGCGCGCCGACCTGCCTCCGCCCCGCGGAGCGGGCTTCGACCTGGTCTGACGTGCGGAA GAGCGTGCCGCGGTCTTACTCGCCATACCGGTAAGACTAGTCGCATCAGCCTCAGATACACCATCTGCAA CACTAGTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3
Genome related info at NCBI

Gene(s) or part of gene(s) amplified:
ORF.. 3457609-3458649	Sequence	Mspyr1_33380 - methyltransferase family protein
ORF.. 3458646-3461315	Sequence	Mspyr1_33390 - DNA segregation ATPase FtsK (Protein is coded in complementary strand; from 3461315 to 3458646)